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Literature summary extracted from

  • Cavalier, M.C.; Kim, S.G.; Neau, D.; Lee, Y.H.
    Molecular basis of the fructose-2,6-bisphosphatase reaction of PFKFB3: transition state and the C-terminal function (2012), Proteins, 80, 1143-1153.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.46 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.46 in complex with D-fructose 6-phosphate, diphosphate, or AlF4, sitting drop vapor diffusion method, using 100 mM Tris-HCl, pH 7.5, 20-25% (w/v) ethylene glycol, 200-400 mM tartaric acid, 5% (v/v) glycerol, and 12% (w/v) polyethylene glycol 4000 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.46 beta-D-fructose 2,6-bisphosphate + H2O Homo sapiens
-
 D-fructose 6-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.46 Homo sapiens Q16875
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.46 Ni-NTA affinity column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.46 beta-D-fructose 2,6-bisphosphate + H2O
-
 Homo sapiens D-fructose 6-phosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.46 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
-
 Homo sapiens
3.1.3.46 PFKFB3 isoform Homo sapiens