Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Singh, S.; Chang, A.; Goff, R.D.; Bingman, C.A.; Grschow, S.; Sherman, D.H.; Phillips, G.N. Jr.; Thorson, J.S.
    Structural characterization of the mitomycin 7-O-methyltransferase (2011), Proteins, 79, 2181-2188.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.316 expression in Escherichia coli as N-terminal polyhistidine-tagged protein Streptomyces lavendulae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.316 hanging drop vapor diffusion method, crystal structures of the mitomycin-7-O-methyltransferase–S-adenosylhomocysteine binary complex and mitomycin-7-O-methyltransferase–S-adenosyl-L-homocysteine–mitomycin A ternary complex at resolutions of 1.9 and 2.3 A, respectively Streptomyces lavendulae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.316 additional information Streptomyces lavendulae the enzyme is involved in the biosynthesis of the quinone-containing antibiotic mitomycin ?
-
 ?
2.1.1.316 additional information Streptomyces lavendulae NRRL 2564 the enzyme is involved in the biosynthesis of the quinone-containing antibiotic mitomycin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.316 Streptomyces lavendulae Q9X5T6
-
-
2.1.1.316 Streptomyces lavendulae NRRL 2564 Q9X5T6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.316
-
 Streptomyces lavendulae

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.316 S-adenosyl-L-methionine + 6-demethylmitomycin A = S-adenosyl-L-homocysteine + mitomycin A (1) Streptomyces lavendulae
a
2.1.1.316 S-adenosyl-L-methionine + 6-demethylmitomycin B = S-adenosyl-L-homocysteine + mitomycin B (2) Streptomyces lavendulae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.316 additional information the enzyme is involved in the biosynthesis of the quinone-containing antibiotic mitomycin Streptomyces lavendulae ?
-
 ?
2.1.1.316 additional information the enzyme catalyzes the 7-O-methylation of both C9beta- and C9alpha-configured 7-hydroxymitomycins Streptomyces lavendulae ?
-
 ?
2.1.1.316 additional information the enzyme is involved in the biosynthesis of the quinone-containing antibiotic mitomycin Streptomyces lavendulae NRRL 2564 ?
-
 ?
2.1.1.316 additional information the enzyme catalyzes the 7-O-methylation of both C9beta- and C9alpha-configured 7-hydroxymitomycins Streptomyces lavendulae NRRL 2564 ?
-
 ?
2.1.1.316 S-adenosyl-L-methionine + 6-demethylmitomycin A
-
 Streptomyces lavendulae S-adenosyl-L-homocysteine + mitomycin A
-
 ?
2.1.1.316 S-adenosyl-L-methionine + 6-demethylmitomycin A
-
 Streptomyces lavendulae NRRL 2564 S-adenosyl-L-homocysteine + mitomycin A
-
 ?
2.1.1.316 S-adenosyl-L-methionine + 6-demethylmitomycin B
-
 Streptomyces lavendulae S-adenosyl-L-homocysteine + mitomycin B
-
 ?
2.1.1.316 S-adenosyl-L-methionine + 6-demethylmitomycin B
-
 Streptomyces lavendulae NRRL 2564 S-adenosyl-L-homocysteine + mitomycin B
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.316 dimer
-
 Streptomyces lavendulae

Synonyms

EC Number Synonyms Comment Organism
2.1.1.316 MmcR
-
 Streptomyces lavendulae

General Information

EC Number General Information Comment Organism
2.1.1.316 physiological function the enzyme is involved in the biosynthesis of the quinone-containing antibiotic mitomycin Streptomyces lavendulae