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Literature summary extracted from

  • Ringel, A.E.; Roman, C.; Wolberger, C.
    Alternate deacylating specificities of the archaeal sirtuins Sir2Af1 and Sir2Af2 (2014), Protein Sci., 23, 1686-1697.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.B41 vapor diffusion at 20°C, crystal structure of the enzyme bound to KGLGKGGA(N6-myristoyl)KRHRKW Archaeoglobus fulgidus
2.3.1.B43 vapor diffusion at 20°C, crystal structure of the enzyme bound to KGLGKGGA(N6-succinyl)KRHRKW Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.B41 Archaeoglobus fulgidus O30124
-
-
2.3.1.B43 Archaeoglobus fulgidus O28597
-
-
2.3.1.B43 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.B41
-
 Archaeoglobus fulgidus
2.3.1.B43
-
 Escherichia coli
2.3.1.B43
-
 Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.B41 additional information no activity with KGLGKGGA(N6-succinyl)KRHRKW Archaeoglobus fulgidus ?
-
 ?
2.3.1.B41 NAD+ + KGLGKGGA(N6-acetyl)KRHRKW the enzyme shows increased reaction velocity with increasing acyl chain length. As compared to its deacetylating activity, Sir2Af2 depropionylates, debutyrylates, and demyristoylates a peptide of the same sequence at 1.5-, 1.9-, and 3.4-fold higher rates, respectively Archaeoglobus fulgidus nicotinamide + KGLGKGGAKRHRKW + 2'-O-acetyl-ADP-ribose
-
 ?
2.3.1.B41 NAD+ + KGLGKGGA(N6-butyryl)KRHRKW the enzyme shows increased reaction velocity with increasing acyl chain length. As compared to its deacetylating activity, Sir2Af2 depropionylates, debutyrylates, and demyristoylates a peptide of the same sequence at 1.5-, 1.9-, and 3.4-fold higher rates, respectively Archaeoglobus fulgidus nicotinamide + KGLGKGGAKRHRKW + 2'-O-butyryl-ADP-ribose
-
 ?
2.3.1.B41 NAD+ + KGLGKGGA(N6-myristoyl)KRHRKW the enzyme shows increased reaction velocity with increasing acyl chain length. As compared to its deacetylating activity, Sir2Af2 depropionylates, debutyrylates, and demyristoylates a peptide of the same sequence at 1.5-, 1.9-, and 3.4-fold higher rates, respectively Archaeoglobus fulgidus nicotinamide + KGLGKGGAKRHRKW + 2'-O-myristoyl-ADP-ribose
-
 ?
2.3.1.B41 NAD+ + KGLGKGGA(N6-propionyl)KRHRKW the enzyme shows increased reaction velocity with increasing acyl chain length. As compared to its deacetylating activity, Sir2Af2 depropionylates, debutyrylates, and demyristoylates a peptide of the same sequence at 1.5-, 1.9-, and 3.4-fold higher rates, respectively Archaeoglobus fulgidus nicotinamide + KGLGKGGAKRHRKW + 2'-O-propionyl-ADP-ribose
-
 ?
2.3.1.B43 NAD+ + KGLGKGGA(N6-acetyl)KRHRKW the rate of desuccinylation is 2.3fold faster than the rate of deacetylation Escherichia coli nicotinamide + KGLGKGGAKRHRKW + 2'-O-acetyl-ADP-ribose
-
 ?
2.3.1.B43 NAD+ + KGLGKGGA(N6-acetyl)KRHRKW the rate of desuccinylation is about 5fold faster than the rate of deacetylation Archaeoglobus fulgidus nicotinamide + KGLGKGGAKRHRKW + 2'-O-acetyl-ADP-ribose
-
 ?
2.3.1.B43 NAD+ + KGLGKGGA(N6-butyryl)KRHRKW the rate of desuccinylation is 5.8fold faster than the rate of deburyrylation Escherichia coli nicotinamide + KGLGKGGAKRHRKW + 2'-O-butyryl-ADP-ribose
-
 ?
2.3.1.B43 NAD+ + KGLGKGGA(N6-butyryl)KRHRKW the rate of desuccinylation is about 10fold faster than the rate of debutyrylation Archaeoglobus fulgidus nicotinamide + KGLGKGGAKRHRKW + 2'-O-butyryl-ADP-ribose
-
 ?
2.3.1.B43 NAD+ + KGLGKGGA(N6-propionyl)KRHRKW the rate of desuccinylation is 3.8fold faster than the rate of depropionylation Escherichia coli nicotinamide + KGLGKGGAKRHRKW + 2'-O-propionyl-ADP-ribose
-
 ?
2.3.1.B43 NAD+ + KGLGKGGA(N6-propionyl)KRHRKW the rate of desuccinylation is about 5fold faster than the rate of depropionylation Archaeoglobus fulgidus nicotinamide + KGLGKGGAKRHRKW + 2'-O-propionyl-ADP-ribose
-
 ?
2.3.1.B43 NAD+ + KGLGKGGA(N6-succinyl)KRHRKW the rate of desuccinylation is 2.3fold faster than the rate of deacetylation Escherichia coli nicotinamide + KGLGKGGAKRHRKW + 2'-O-succinyl-ADP-ribose
-
 ?
2.3.1.B43 NAD+ + KGLGKGGA(N6-succinyl)KRHRKW the rate of desuccinylation is about 5fold faster than the rate of deacetylation Archaeoglobus fulgidus nicotinamide + KGLGKGGAKRHRKW + 2'-O-succinyl-ADP-ribose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.B41 Sir2Af2
-
 Archaeoglobus fulgidus
2.3.1.B43 CobB
-
 Escherichia coli
2.3.1.B43 Sir2Af1
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.B41 50
-
 assay at Archaeoglobus fulgidus
2.3.1.B43 37
-
 assay at Escherichia coli
2.3.1.B43 50
-
 assay at Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.B41 8
-
 assay at Archaeoglobus fulgidus
2.3.1.B43 8
-
 assay at Escherichia coli
2.3.1.B43 8
-
 assay at Archaeoglobus fulgidus