Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pais, T.M.; Lamosa, P.; Matzapetakis, M.; Turner, D.L.; Santos, H.
    Mannosylglycerate stabilizes staphylococcal nuclease with restriction of slow beta-sheet motions (2012), Protein Sci., 21, 1126-1137.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.1.31.1 P117G/H124L/S128A site-directed mutagenesis Staphylococcus aureus

General Stability

EC Number General Stability Organism
3.1.31.1 correlation between the magnitude of protein stabilization and the restriction of fast backbone motions, mannosylglycerate restricts local motions in addition to the global motions of the protein. Unfolding/folding pathway remain undisturbed in the presence of mannosylglycerate but the solute shows a specific effect on the local motions of beta-sheet residues Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.31.1 additional information
-
 additional information P117G/H124L/S128A mutant enzyme thermodynamics, overview Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
3.1.31.1 Staphylococcus aureus
-
-
-

Subunits

EC Number Subunits Comment Organism
3.1.31.1 More solution structure of the hyperstable P117G/H124L/S128A mutant enzyme variant, overview Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
3.1.31.1 SNase
-
 Staphylococcus aureus
3.1.31.1 staphylococcal nuclease
-
 Staphylococcus aureus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.31.1 additional information
-
 different concentrations of mannosylglycerate or presence of urea at 0.25M show no correlation with changes in the thermodynamic stability of the P117G/H124L/S128A mutant enzyme Staphylococcus aureus

General Information

EC Number General Information Comment Organism
3.1.31.1 additional information mannosylglycerate preferentially affects specific structural elements of the P117G/H124L/S128A mutant enzyme, structure analysis, overview Staphylococcus aureus