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Literature summary extracted from

  • Vijayakumar, K.R.; Gowda, L.R.
    Rice (Oryza sativa) lipase: molecular cloning, functional expression and substrate specificity (2013), Protein Expr. Purif., 88, 67-79.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 expressed in Escherichia coli (DE3)pLysS, RIL(DE3)pLysS, Rosetta(DE3)pLysS and Origami(DE3)pLysS cells (insoluble inclusion bodies) or in Pichia pastoris X-33 cells (secretory protein) Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 0.04533
-
 4-nitrophenyl acetate native enzyme, at pH 7.4 and 25°C Oryza sativa
3.1.1.3 0.1089
-
 Triacetin native enzyme, at pH 7.4 and 25°C Oryza sativa
3.1.1.3 0.1408
-
 Triacetin recombinant enzyme, at pH 7.4 and 25°C Oryza sativa
3.1.1.3 2.766
-
 4-nitrophenyl acetate recombinant enzyme, at pH 7.4 and 25°C Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 36680
-
 x * 36680, calculated from amino acid sequence Oryza sativa
3.1.1.3 40000
-
 x * 40000, SDS-PAGE Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Oryza sativa
-
 subspecies Indica, variant IR64
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 DEAE-Sepharose column chromatography Oryza sativa

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.1.3 using 0.05 M phosphate buffer, 2 M urea, 0.5 M oxidized glutathione and 0.001 M reduced glutathione, pH 7.4, at 4°C Oryza sativa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 292.4
-
 after 3.41fold purification, with triacetin as substrate, at pH 7.4 and 25°C Oryza sativa
3.1.1.3 998
-
 after 3.41fold purification, with triacetin as substrate, at pH 7.4 and 25°C Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 1-naphthyl acetate + H2O 36.9% activity compared to rice bran oil Oryza sativa 1-naphthol + acetate
-
 ?
3.1.1.3 1-naphthyl butyrate + H2O 18.8% activity compared to rice bran oil Oryza sativa 1-naphthol + butyrate
-
 ?
3.1.1.3 4-nitrophenyl acetate + H2O 0.2% activity compared to rice bran oil Oryza sativa 4-nitrophenol + acetate
-
 ?
3.1.1.3 coconut oil + H2O 47.9% activity compared to rice bran oil Oryza sativa ?
-
 ?
3.1.1.3 olive oil + H2O 95.3% activity compared to rice bran oil Oryza sativa ?
-
 ?
3.1.1.3 palm oil + H2O 77.7% activity compared to rice bran oil Oryza sativa ?
-
 ?
3.1.1.3 rice bran oil 100% activity Oryza sativa ?
-
 ?
3.1.1.3 sunflower oil + H2O 91.7% activity compared to rice bran oil Oryza sativa ?
-
 ?
3.1.1.3 triacetin + H2O 120% activity compared to rice bran oil Oryza sativa ?
-
 ?
3.1.1.3 tributyrin + H2O 92.6% activity compared to rice bran oil Oryza sativa ?
-
 ?
3.1.1.3 trimyristin + H2O 90.1% activity compared to rice bran oil Oryza sativa ?
-
 ?
3.1.1.3 tripalmitin + H2O 82.1% activity compared to rice bran oil Oryza sativa ?
-
 ?
3.1.1.3 tristearin + H2O 72.9% activity compared to rice bran oil Oryza sativa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 40000, SDS-PAGE Oryza sativa
3.1.1.3 ? x * 36680, calculated from amino acid sequence Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 acylglycerol acylhydrolase
-
 Oryza sativa
3.1.1.3 lipase
-
 Oryza sativa
3.1.1.3 RBL
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 25
-
-
 Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.3 0.1
-
 Triacetin native enzyme, at pH 7.4 and 25°C Oryza sativa
3.1.1.3 0.32
-
 Triacetin recombinant enzyme, at pH 7.4 and 25°C Oryza sativa
3.1.1.3 0.36
-
 4-nitrophenyl acetate native enzyme, at pH 7.4 and 25°C Oryza sativa
3.1.1.3 0.44
-
 4-nitrophenyl acetate recombinant enzyme, at pH 7.4 and 25°C Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 7.4
-
-
 Oryza sativa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 6 7.4 remains stable Oryza sativa