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Literature summary extracted from

  • Wang, H.; Li, X.; Ma, Y.; Song, J.
    Characterization and high-level expression of a metagenome-derived alkaline pectate lyase in recombinant Escherichia coli (2014), Process Biochem., 49, 69-76.
No PubMed abstract available

Application

EC Number Application Comment Organism
4.2.2.2 environmental protection alkaline pectate lyases play an important role in mild and eco-friendly bioscouring pretreatment processes in the textile industry. So far, only a few pectate lyases can be applied in industrial-scale production, and many of them exhibit high production cost, low activity, and/or do not meet the treatment requirements. Recombinant PelB has with promising properties for use in bioscouring in the textile pretreatment process and is a potential enzyme for industrial applications uncultured bacterium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.2 gene pelB, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) uncultured bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.2 Ba2+ strong inhibition at 0.5-1 mM uncultured bacterium
4.2.2.2 Cu2+ strong inhibition at 1 mM uncultured bacterium
4.2.2.2 Fe2+ strong inhibition at 1 mM uncultured bacterium
4.2.2.2 Mg2+ strong inhibition at 1 mM uncultured bacterium
4.2.2.2 Mn2+ strong inhibition at 0.5-1 mM uncultured bacterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.2 Ca2+ required, activates 5.8fold at 0.5 mM uncultured bacterium
4.2.2.2 Cu2+ activates slightly at 0.5 mM uncultured bacterium
4.2.2.2 K+ activates slightly at 0.5-1 mM uncultured bacterium
4.2.2.2 Mg2+ activates slightly at 0.5 mM uncultured bacterium
4.2.2.2 Triton X-100 activates slightly at 0.5-1 mM uncultured bacterium
4.2.2.2 Tween-20 activates slightly at 1 mM uncultured bacterium
4.2.2.2 Zn2+ activates slightly at 0.5-1 mM uncultured bacterium

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.2 uncultured bacterium S5UBI8 gene pelB cloned from the metagenomic DNA of alkaline environment soils
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.2 recombinant His-tagged enzyme 2.47fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography uncultured bacterium

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.2.2 additional information high cell-density cultivation in 7-L bioreactor, expression profile over time, overview uncultured bacterium
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.2 614.7
-
 purified recombinant His-tagged enzyme, 50-70% methylated pectin, pH 9.8, 45°C uncultured bacterium
4.2.2.2 668.2
-
 purified recombinant His-tagged enzyme, over 80% methylated pectin, pH 9.8, 45°C uncultured bacterium
4.2.2.2 1984
-
 purified recombinant His-tagged enzyme, unmethylated polygalacturonic acid, pH 9.8, 45°C uncultured bacterium
4.2.2.2 2096
-
 purified recombinant His-tagged enzyme, 20-34% methylated polygalacturonic acid, pH 9.8, 45°C uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.2 additional information PelB is an endo-acting lyase and shows high cleavage capability on a broad range of substrates of natural methylated pectin, substrate specificity, overview uncultured bacterium ?
-
 ?
4.2.2.2 pectin methylated at low-degree uncultured bacterium ?
-
 ?
4.2.2.2 polygalacturonic acid best substrate uncultured bacterium ? final major end products are dimers, trimers, and tetramers of unsaturated galacturonic acid, PelB does not produce any monomeric GalpA ?

Synonyms

EC Number Synonyms Comment Organism
4.2.2.2 alkaline pectate lyase
-
 uncultured bacterium
4.2.2.2 PelB
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.2 55
-
-
 uncultured bacterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.2 35 70 activity range uncultured bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.2 45
-
 purified recombinant enzyme, 2 h, 64.9% activity remaining, half-life is 360 min uncultured bacterium
4.2.2.2 50
-
 purified recombinant enzyme, half-life is 88 min uncultured bacterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.2.2 750
-
 Pectin 50-70% methylated substrate, pH 9.8, 45°C, recombinant enzyme uncultured bacterium
4.2.2.2 1882
-
 Pectin over 80% methylated substrate, pH 9.8, 45°C, recombinant enzyme uncultured bacterium
4.2.2.2 2295
-
 polygalacturonic acid 20-34% methylated substrate, pH 9.8, 45°C, recombinant enzyme uncultured bacterium
4.2.2.2 6092
-
 polygalacturonic acid unmethylated substrate, pH 9.8, 45°C, recombinant enzyme uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.2 8.1
-
 optimum 1, about 70% of maximal activity uncultured bacterium
4.2.2.2 9.8
-
 optimum 2, maximal activity uncultured bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.2 4 12 activity range uncultured bacterium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.2 4 10.4 purified recombinant enzyme, about 50% activity within this range uncultured bacterium