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Literature summary extracted from

  • Larrouy-Maumus, G.; Biswas, T.; Hunt, D.M.; Kelly, G.; Tsodikov, O.V.; de Carvalho, L.P.
    Discovery of a glycerol 3-phosphate phosphatase reveals glycerophospholipid polar head recycling in Mycobacterium tuberculosis (2013), Proc. Natl. Acad. Sci. USA, 110, 11320-11325.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.21 0.89
-
 DL-glycerol 3-phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis
3.1.3.21 1.1
-
 L-glycerol 3-phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis
3.1.3.21 1.3
-
 D-ribulose 5-phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis
3.1.3.21 2.01
-
 Glycerol 2-phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis
3.1.3.21 19.3
-
 4-nitrophenyl phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.21 Co2+ Mn2+ supports 8fold higher catalytic efficiency than Mg2+ Mycobacterium tuberculosis
3.1.3.21 Mg2+
-
 Mycobacterium tuberculosis
3.1.3.21 Mn2+ Mn2+ supports 4fold higher catalytic efficiency than Mg2+ Mycobacterium tuberculosis
3.1.3.21 Ni2+ for Ni2+, the catalytic efficiency is approximately half of that for Mg2+ Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.21 DL-glycerol 3-phosphate + H2O Mycobacterium tuberculosis preferred substrate glycerol + phosphate
-
 ?
3.1.3.21 DL-glycerol 3-phosphate + H2O Mycobacterium tuberculosis H37Rv preferred substrate glycerol + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.21 Mycobacterium tuberculosis O33194
-
-
3.1.3.21 Mycobacterium tuberculosis H37Rv O33194
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.21 4-nitrophenyl phosphate + H2O
-
 Mycobacterium tuberculosis 4-nitrophenol + phosphate
-
 ?
3.1.3.21 4-nitrophenyl phosphate + H2O
-
 Mycobacterium tuberculosis H37Rv 4-nitrophenol + phosphate
-
 ?
3.1.3.21 D-ribulose 5-phosphate + H2O
-
 Mycobacterium tuberculosis D-ribulose + phosphate
-
 ?
3.1.3.21 D-ribulose 5-phosphate + H2O
-
 Mycobacterium tuberculosis H37Rv D-ribulose + phosphate
-
 ?
3.1.3.21 DL-glycerol 3-phosphate + H2O preferred substrate Mycobacterium tuberculosis glycerol + phosphate
-
 ?
3.1.3.21 DL-glycerol 3-phosphate + H2O preferred substrate Mycobacterium tuberculosis H37Rv glycerol + phosphate
-
 ?
3.1.3.21 glycerol 2-phosphate + H2O
-
 Mycobacterium tuberculosis glycerol + phosphate
-
 ?
3.1.3.21 glycerol 2-phosphate + H2O
-
 Mycobacterium tuberculosis H37Rv glycerol + phosphate
-
 ?
3.1.3.21 L-glycerol 3-phosphate + H2O
-
 Mycobacterium tuberculosis glycerol + phosphate
-
 ?
3.1.3.21 additional information NagD, UMP and GMP are not substrates Mycobacterium tuberculosis ?
-
 ?
3.1.3.21 additional information NagD, UMP and GMP are not substrates Mycobacterium tuberculosis H37Rv ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.21 D,L-glycerol 3-phosphate phosphatase
-
 Mycobacterium tuberculosis
3.1.3.21 G3P phosphatase
-
 Mycobacterium tuberculosis
3.1.3.21 GPP
-
 Mycobacterium tuberculosis
3.1.3.21 HADSF phosphatase
-
 Mycobacterium tuberculosis
3.1.3.21 Rv1692
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.21 0.02
-
 L-glycerol 3-phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis
3.1.3.21 0.18
-
 D-ribulose 5-phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis
3.1.3.21 0.18
-
 Glycerol 2-phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis
3.1.3.21 0.77
-
 DL-glycerol 3-phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis
3.1.3.21 1
-
 4-nitrophenyl phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
3.1.3.21 metabolism Rv1692 is the final enzyme involved in glycerophospholipid recycling/catabolism Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.21 0.02
-
 L-glycerol 3-phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis
3.1.3.21 0.05
-
 4-nitrophenyl phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis
3.1.3.21 0.09
-
 Glycerol 2-phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis
3.1.3.21 0.14
-
 D-ribulose 5-phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis
3.1.3.21 0.86
-
 DL-glycerol 3-phosphate in Tris-HCl 50 mM at pH 7.5, at 22°C Mycobacterium tuberculosis