EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.17.99.6 | Cobalamin | stimulates | Escherichia coli | |
1.17.99.6 | Cobalamin | stimulates | Bacillus subtilis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.17.99.6 | expression in Escherichia coli | Bacillus subtilis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.99.6 | epoxyqueuosine34 in tRNA + reduced acceptor | Escherichia coli | the enzyme catalyses the last step in the bacterial biosynthetic pathway to queuosine, the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn | queuosine34 in tRNA + acceptor + H2O | - |
? | |
1.17.99.6 | epoxyqueuosine34 in tRNA + reduced acceptor | Bacillus subtilis | the enzyme catalyses the last step in the bacterial biosynthetic pathway to queuosine, the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn | queuosine34 in tRNA + acceptor + H2O | - |
? | |
1.17.99.6 | epoxyqueuosine34 in tRNA + reduced acceptor | Bacillus subtilis 168 | the enzyme catalyses the last step in the bacterial biosynthetic pathway to queuosine, the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn | queuosine34 in tRNA + acceptor + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.17.99.6 | Bacillus subtilis | P97030 | - |
- |
1.17.99.6 | Bacillus subtilis 168 | P97030 | - |
- |
1.17.99.6 | Escherichia coli | P39288 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.17.99.6 | - |
Escherichia coli |
1.17.99.6 | - |
Bacillus subtilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.99.6 | epoxyqueuosine34 in tRNA + reduced acceptor | the enzyme catalyses the last step in the bacterial biosynthetic pathway to queuosine, the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn | Escherichia coli | queuosine34 in tRNA + acceptor + H2O | - |
? | |
1.17.99.6 | epoxyqueuosine34 in tRNA + reduced acceptor | the enzyme catalyses the last step in the bacterial biosynthetic pathway to queuosine, the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn | Bacillus subtilis | queuosine34 in tRNA + acceptor + H2O | - |
? | |
1.17.99.6 | epoxyqueuosine34 in tRNA + reduced acceptor | the enzyme catalyses the last step in the bacterial biosynthetic pathway to queuosine, the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn | Bacillus subtilis 168 | queuosine34 in tRNA + acceptor + H2O | - |
? | |
1.17.99.6 | epoxyqueuosine34 in tRNA + reduced methyl viologen | conversion of epoxyqueuosine to queuoseine is carried out using either a 17-mer oQ-containing RNA stem loop | Escherichia coli | queuosine34 in tRNA + methyl viologen + H2O | - |
? | |
1.17.99.6 | epoxyqueuosine34 in tRNA + reduced methyl viologen | the recombinant Bacillus subtilis enzyme catalyzes the conversion of epoxyqueuosine to queuoseine in a synthetic substrate, as well as undermodified RNA isolated from an oQ reductase knockout strain | Bacillus subtilis | queuosine34 in tRNA + methyl viologen + H2O | - |
? | |
1.17.99.6 | epoxyqueuosine34 in tRNA + reduced methyl viologen | the recombinant Bacillus subtilis enzyme catalyzes the conversion of epoxyqueuosine to queuoseine in a synthetic substrate, as well as undermodified RNA isolated from an oQ reductase knockout strain | Bacillus subtilis 168 | queuosine34 in tRNA + methyl viologen + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.17.99.6 | oQ reductase | - |
Escherichia coli |
1.17.99.6 | oQ reductase | - |
Bacillus subtilis |
1.17.99.6 | QueG | - |
Escherichia coli |
1.17.99.6 | QueG | - |
Bacillus subtilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.17.99.6 | 7.2 | - |
assay at | Escherichia coli |
1.17.99.6 | 7.2 | - |
assay at | Bacillus subtilis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.17.99.6 | metabolism | the enzyme catalyses the last step in the bacterial biosynthetic pathway to queuosine, the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn | Escherichia coli |
1.17.99.6 | metabolism | the enzyme catalyses the last step in the bacterial biosynthetic pathway to queuosine, the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn | Bacillus subtilis |