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Literature summary extracted from

  • Rangachary, L.; Rajagopalan, R.P.; Singh, T.M.; Krishnan, M.H.
    Purification and characterization of DDT-dehydrohalogenase from Pseudomonas putida T5 (2012), Prep. Biochem. Biotechnol., 42, 60-76.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.5.1.1 0.037
-
 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane pH 7.0, 37°C Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.5.1.1 Ca2+ a metalloprotein containing Zn2+, Mg2+, and Ca2+ ions Pseudomonas putida
4.5.1.1 Mg2+ a metalloprotein containing Zn2+, Mg2+, and Ca2+ ions Pseudomonas putida
4.5.1.1 Zn2+ a metalloprotein containing Zn2+, Mg2+, and Ca2+ ions Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.5.1.1 37000
-
 about, native PAGE Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.5.1.1 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane Pseudomonas putida
-
 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride
-
 ?
4.5.1.1 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane Pseudomonas putida T5
-
 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.5.1.1 Pseudomonas putida
-
-
-
4.5.1.1 Pseudomonas putida T5
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.5.1.1 glycoprotein a glycoprotein with mannose forming the backbone Pseudomonas putida

Purification (Commentary)

EC Number Purification (Comment) Organism
4.5.1.1 native enzyme from strain T5 to homogeneity Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.5.1.1 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
-
 Pseudomonas putida 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride
-
 ?
4.5.1.1 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane
-
 Pseudomonas putida T5 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + chloride
-
 ?

Subunits

EC Number Subunits Comment Organism
4.5.1.1 monomer 1 * 37000, SDS-PAGE Pseudomonas putida
4.5.1.1 More mass spectrometric sequence canalysis Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
4.5.1.1 DDT-dehydrohalogenase
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.5.1.1 37
-
-
 Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.5.1.1 37
-
 purified native enzyme, pH 7.0, stable for 4-5 h Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.5.1.1 0.113
-
 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane pH 7.0, 37°C Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5.1.1 7
-
-
 Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.5.1.1 7
-
 purified native enzyme, 37°C, stable for 4-5 h Pseudomonas putida

General Information

EC Number General Information Comment Organism
4.5.1.1 additional information serine and tryptophan appear to be involved at the active site Pseudomonas putida
4.5.1.1 physiological function the enzyme is involved in the catalytic degradation of 4,4'-DDT by eliminating either HCl or Cl- to form DDD/DDE Pseudomonas putida