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Literature summary extracted from

  • Farelli, J.D.; Galvin, B.D.; Li, Z.; Liu, C.; Aono, M.; Garland, M.; Hallett, O.E.; Causey, T.B.; Ali-Reynolds, A.; Saltzberg, D.J.; Carlow, C.K.; Dunaway-Mariano, D.; Allen, K.N.
    Structure of the trehalose-6-phosphate phosphatase from Brugia malayi reveals key design principles for anthelmintic drugs (2014), PLoS Pathog., 10, e1004245.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.12 expressed in Escherichia coli T7 Express cells Brugia malayi

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.12 hanging drop vapor diffusion method, using 33% (w/v) PEG 300, 0.1 M sodium citrate pH 5.0, 13% ethylene glycol and 10 mM CoCl2 Brugia malayi

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.12 D213A inactive Brugia malayi
3.1.3.12 D215A inactive Brugia malayi
3.1.3.12 D336A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Brugia malayi
3.1.3.12 D378A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Brugia malayi
3.1.3.12 DELTA59 the mutant shows increased catalytic efficiency compared to the wild type enzyme Brugia malayi
3.1.3.12 E384A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Brugia malayi
3.1.3.12 E386A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Brugia malayi
3.1.3.12 K334A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Brugia malayi
3.1.3.12 N228A the mutant shows increased catalytic efficiency compared to the wild type enzyme Brugia malayi
3.1.3.12 Q332A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Brugia malayi
3.1.3.12 R337A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Brugia malayi
3.1.3.12 S329A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Brugia malayi
3.1.3.12 T339A the mutant shows increased catalytic efficiency compared to the wild type enzyme Brugia malayi
3.1.3.12 W280A the mutant shows increased catalytic efficiency compared to the wild type enzyme Brugia malayi
3.1.3.12 Y221A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Brugia malayi
3.1.3.12 Y225A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Brugia malayi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.12 D-glucose 6-sulfate
-
 Brugia malayi
3.1.3.12 trehalose
-
 Brugia malayi
3.1.3.12 trehalose 6-sulfate competitive inhibitor Brugia malayi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.12 0.04
-
 trehalose 6-phosphate mutant enzyme N228A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 0.046
-
 trehalose 6-phosphate mutant enzyme D336A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 0.082
-
 trehalose 6-phosphate mutant enzyme E386A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 0.1
-
 trehalose 6-phosphate mutant enzyme E384A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 0.15
-
 trehalose 6-phosphate mutant enzyme W280A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 0.15
-
 trehalose 6-phosphate mutant enzyme Y225A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 0.2
-
 trehalose 6-phosphate mutant enzyme Q332A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 0.2
-
 trehalose 6-phosphate mutant enzyme T339A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 0.25
-
 trehalose 6-phosphate mutant enzyme S329A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 0.26
-
 trehalose 6-phosphate mutant enzyme DELTA59, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 0.27
-
 trehalose 6-phosphate mutant enzyme D378A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 0.33
-
 trehalose 6-phosphate mutant enzyme Y221A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 0.36
-
 trehalose 6-phosphate wild type enzyme, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 0.4
-
 trehalose 6-phosphate mutant enzyme R337A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 0.5
-
 trehalose 6-phosphate mutant enzyme K334A, at pH 7.5 and 22°C Brugia malayi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.12 Mg2+ required Brugia malayi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.12 trehalose 6-phosphate + H2O Brugia malayi
-
 trehalose + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.12 Brugia malayi A8NS89
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.12 HiTrap Q column chromatography Brugia malayi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.12 trehalose 6-phosphate + H2O
-
 Brugia malayi trehalose + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.12 GOB-1
-
 Brugia malayi
3.1.3.12 T6PP
-
 Brugia malayi
3.1.3.12 trehalose 6-phosphate phosphatase
-
 Brugia malayi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.12 0.06
-
 trehalose 6-phosphate mutant enzyme K334A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 0.094
-
 trehalose 6-phosphate mutant enzyme D336A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 0.22
-
 trehalose 6-phosphate mutant enzyme Q332A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 0.28
-
 trehalose 6-phosphate mutant enzyme R337A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 1
-
 trehalose 6-phosphate mutant enzyme Y221A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 2 8 trehalose 6-phosphate mutant enzyme DELTA59, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 2
-
 trehalose 6-phosphate mutant enzyme E386A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 3
-
 trehalose 6-phosphate mutant enzyme E384A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 4
-
 trehalose 6-phosphate mutant enzyme S329A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 6
-
 trehalose 6-phosphate mutant enzyme Y225A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 11
-
 trehalose 6-phosphate mutant enzyme D378A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 15
-
 trehalose 6-phosphate mutant enzyme T339A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 18
-
 trehalose 6-phosphate mutant enzyme N228A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 19
-
 trehalose 6-phosphate mutant enzyme W280A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 24
-
 trehalose 6-phosphate wild type enzyme, at pH 7.5 and 22°C Brugia malayi

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.12 0.082
-
 trehalose 6-sulfate wild type enzyme, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 10
-
 trehalose Ki above 10 mM, wild type enzyme, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 10
-
 D-glucose 6-sulfate Ki above 10 mM, wild type enzyme, at pH 7.5 and 22°C Brugia malayi

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.12 0.11
-
 trehalose 6-phosphate mutant enzyme K334A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 0.2
-
 trehalose 6-phosphate mutant enzyme D336A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 0.55
-
 trehalose 6-phosphate mutant enzyme Q332A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 1.5
-
 trehalose 6-phosphate mutant enzyme R337A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 3
-
 trehalose 6-phosphate mutant enzyme Y221A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 15
-
 trehalose 6-phosphate mutant enzyme S329A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 28
-
 trehalose 6-phosphate mutant enzyme E386A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 30
-
 trehalose 6-phosphate mutant enzyme E384A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 36
-
 trehalose 6-phosphate mutant enzyme Y225A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 39
-
 trehalose 6-phosphate mutant enzyme D378A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 69
-
 trehalose 6-phosphate wild type enzyme, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 77
-
 trehalose 6-phosphate mutant enzyme T339A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 110
-
 trehalose 6-phosphate mutant enzyme DELTA59, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 120
-
 trehalose 6-phosphate mutant enzyme W280A, at pH 7.5 and 22°C Brugia malayi
3.1.3.12 450
-
 trehalose 6-phosphate mutant enzyme N228A, at pH 7.5 and 22°C Brugia malayi