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Literature summary extracted from

  • Guo, X.; Shi, Y.; Sheng, J.; Wang, F.
    A novel hyaluronidase produced by Bacillus sp. A50 (2014), PLoS ONE, 9, e94156.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.36 Cu2+ strong inhibition at 100 mM Bacillus sp. (in: Bacteria)
3.2.1.36 deferoxamine mesylate
-
 Bacillus sp. (in: Bacteria)
3.2.1.36 EDTA
-
 Bacillus sp. (in: Bacteria)
3.2.1.36 EGTA
-
 Bacillus sp. (in: Bacteria)
3.2.1.36 SDS complete inhibition at any concentrations Bacillus sp. (in: Bacteria)
3.2.1.36 Triton X-100 moderately inhibits enzyme activity by 3%-30% at different concentrations Bacillus sp. (in: Bacteria)
3.2.1.36 Tween 80 moderately inhibits enzyme activity by 3%-30% at different concentrations Bacillus sp. (in: Bacteria)
3.2.1.36 Zn2+ strong inhibition at 50 mM Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.36 Ca2+ 154% activity at 100 mM Bacillus sp. (in: Bacteria)
3.2.1.36 Mg2+ has positive effect on the activity Bacillus sp. (in: Bacteria)
3.2.1.36 Ni2+ has positive effect on the activity Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.36 120000
-
 2 * 120000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.36 Bacillus sp. (in: Bacteria)
-
-
-
3.2.1.36 Bacillus sp. (in: Bacteria) A50
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.36 ammonium sulfate precipitation, DEAE Sepharose column chromatography, and Superdex 200 gel filtration Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.36 4.86
-
 culture supernatant, at pH 6.5 and 44°C Bacillus sp. (in: Bacteria)
3.2.1.36 102.1
-
 after 21.02fold purification, at pH 6.5 and 44°C Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.36 chondroitin sulfate A + H2O 39% activity compared to hyaluronan Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.36 chondroitin sulfate A + H2O 39% activity compared to hyaluronan Bacillus sp. (in: Bacteria) A50 ?
-
 ?
3.2.1.36 chondroitin sulfate C + H2O 13.5% activity compared to hyaluronan Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.36 chondroitin sulfate C + H2O 13.5% activity compared to hyaluronan Bacillus sp. (in: Bacteria) A50 ?
-
 ?
3.2.1.36 chondroitin sulfate D + H2O 37.1% activity compared to hyaluronan Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.36 chondroitin sulfate D + H2O 37.1% activity compared to hyaluronan Bacillus sp. (in: Bacteria) A50 ?
-
 ?
3.2.1.36 hyaluronan + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.36 hyaluronan + H2O
-
 Bacillus sp. (in: Bacteria) A50 ?
-
 ?
3.2.1.36 additional information no activity with dermatan sulfate, heparin sodium, heparan sulfate, sodium alginate, chitosan, chitin, sodium carboxymethyl cellulose, dextran, carboxymethyl dextran, xanthan gum, gellan gum, soluble starch, carrageenan, Konjac glucomannan, synanthrin, gumacabic powder, glycogen, or pectin Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.36 additional information no activity with dermatan sulfate, heparin sodium, heparan sulfate, sodium alginate, chitosan, chitin, sodium carboxymethyl cellulose, dextran, carboxymethyl dextran, xanthan gum, gellan gum, soluble starch, carrageenan, Konjac glucomannan, synanthrin, gumacabic powder, glycogen, or pectin Bacillus sp. (in: Bacteria) A50 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.36 ? 2 * 120000, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

EC Number Synonyms Comment Organism
3.2.1.36 HAase-B
-
 Bacillus sp. (in: Bacteria)
3.2.1.36 hyaluronidase
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.36 44
-
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.36 40 60 the activity of the enzyme remains high and relatively stable between 40°C and 44°C, with 25% of maximum activity at 46°C. At 60°C or higher, the enzyme loses its activity completely Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.36 40 60 after incubation at 40°C or 45°C for 120 min, the enzyme shows little change with its activity, but its stability is very low at 55°C. The activity is reduced by 99% after incubation at 55°C for 10 min Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.36 6.5
-
-
 Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.36 4 9 there is no detectable activity at pH 4.0 and pH 9.0 Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.36 5.9 6 the enzyme is most stable at pH 5.0-6.0 and retains more than 90% activity after incubation at this pH range for 2 h Bacillus sp. (in: Bacteria)