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Literature summary extracted from

  • Ratia, K.; Light, S.H.; Antanasijevic, A.; Anderson, W.F.; Caffrey, M.; Lavie, A.
    Discovery of selective inhibitors of the Clostridium difficile dehydroquinate dehydratase (2014), PLoS ONE, 9, e89356.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.10 expressed in Escherichia coli BL21(DE3) cells Clostridioides difficile

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.10 2,5-dichloro-N-(4-phenyl-1,3-thiazol-2-yl)benzenesulfonamide
-
 Clostridioides difficile
4.2.1.10 2,5-dichloro-N-(6-methoxy-1,3-benzothiazol-2-yl)benzenesulfonamide
-
 Clostridioides difficile
4.2.1.10 2-[(4,5,6,7-tetrahydro-1-benzothiophen-3-ylcarbonyl)amino]benzoic acid
-
 Clostridioides difficile

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.10 3-dehydroquinate Clostridioides difficile
-
 3-dehydroshikimate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.10 Clostridioides difficile
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.10 His-Trap HP Ni Sepharose column chromatography, and S-200 gel filtration Clostridioides difficile

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.10 3-dehydroquinate
-
 Clostridioides difficile 3-dehydroshikimate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.10 aroD
-
 Clostridioides difficile
4.2.1.10 dehydroquinate dehydratase
-
 Clostridioides difficile
4.2.1.10 DHQD
-
 Clostridioides difficile

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.2.1.10 0.031
-
 in 50 mM HEPES, pH 7.5, at 22°C Clostridioides difficile 2-[(4,5,6,7-tetrahydro-1-benzothiophen-3-ylcarbonyl)amino]benzoic acid
4.2.1.10 0.034
-
 in 50 mM HEPES, pH 7.5, at 22°C Clostridioides difficile 2,5-dichloro-N-(4-phenyl-1,3-thiazol-2-yl)benzenesulfonamide
4.2.1.10 0.035
-
 in 50 mM HEPES, pH 7.5, at 22°C Clostridioides difficile 2,5-dichloro-N-(6-methoxy-1,3-benzothiazol-2-yl)benzenesulfonamide