BRENDA - Enzyme Database

Characterization of a soluble phosphatidic acid phosphatase in bitter melon (Momordica charantia)

Cao, H.; Sethumadhavan, K.; Grimm, C.C.; Ullah, A.H.; PLoS ONE 9, e106403 (2014)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
CuSO4
-
Momordica charantia
3.1.3.4
FeSO4
-
Momordica charantia
3.1.3.4
additional information
presence of EDTA or EGTA does not significantly affect enzyme activity
Momordica charantia
3.1.3.4
Na3VO4
2 mM, about 90% inhibition
Momordica charantia
3.1.3.4
NaF
-
Momordica charantia
3.1.3.4
Triton X-100
-
Momordica charantia
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
0.5954
-
1,2-diacyl-sn-glycerol-3-phosphate
pH 6.5, 53°C
Momordica charantia
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Co(NO3)2
0.3 mM, about 3fold stimulation
Momordica charantia
3.1.3.4
MnSO4
0.3 mM, about 3fold stimulation
Momordica charantia
3.1.3.4
additional information
activity is independent of Mg2+
Momordica charantia
3.1.3.4
ZnSO4
0.3 mM, about 2.5fold stimulation
Momordica charantia
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Momordica charantia
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.4
partial
Momordica charantia
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
cotyledon
developing cotyledon
Momordica charantia
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.4
312
-
pH 6.5, 53°C
Momordica charantia
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
1,2-diacyl-sn-glycerol-3-phosphate + H2O
-
730770
Momordica charantia
1,2-diacyl-sn-glycerol + phosphate
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.3.4
53
60
-
Momordica charantia
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
6.5
-
-
Momordica charantia
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
CuSO4
-
Momordica charantia
3.1.3.4
FeSO4
-
Momordica charantia
3.1.3.4
additional information
presence of EDTA or EGTA does not significantly affect enzyme activity
Momordica charantia
3.1.3.4
Na3VO4
2 mM, about 90% inhibition
Momordica charantia
3.1.3.4
NaF
-
Momordica charantia
3.1.3.4
Triton X-100
-
Momordica charantia
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
0.5954
-
1,2-diacyl-sn-glycerol-3-phosphate
pH 6.5, 53°C
Momordica charantia
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Co(NO3)2
0.3 mM, about 3fold stimulation
Momordica charantia
3.1.3.4
MnSO4
0.3 mM, about 3fold stimulation
Momordica charantia
3.1.3.4
additional information
activity is independent of Mg2+
Momordica charantia
3.1.3.4
ZnSO4
0.3 mM, about 2.5fold stimulation
Momordica charantia
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
partial
Momordica charantia
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
cotyledon
developing cotyledon
Momordica charantia
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.4
312
-
pH 6.5, 53°C
Momordica charantia
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
1,2-diacyl-sn-glycerol-3-phosphate + H2O
-
730770
Momordica charantia
1,2-diacyl-sn-glycerol + phosphate
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.3.4
53
60
-
Momordica charantia
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
6.5
-
-
Momordica charantia