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Literature summary extracted from

  • Uraji, M.; Arima, J.; Inoue, Y.; Harazono, K.; Hatanaka, T.
    Application of two newly identified and characterized feruloyl esterases from Streptomyces sp. in the enzymatic production of ferulic acid from agricultural biomass (2014), PLoS ONE, 9, e104584.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.73 Co2+
-
 Streptomyces cinnamoneus
3.1.1.73 Fe3+ strong inhibition of isozyme R18, weak inhibition of isozyme R43 Streptomyces cinnamoneus
3.1.1.73 Ni2+
-
 Streptomyces cinnamoneus
3.1.1.73 PMSF
-
 Streptomyces cinnamoneus
3.1.1.73 Zn2+ strong inhibition of both isozymes Streptomyces cinnamoneus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.17
-
 4-nitrophenyl butyrate isozyme R43, pH 7.0, 50°C Streptomyces cinnamoneus
3.1.1.73 0.54
-
 methyl sinapinate isozyme R43, pH 7.0, 50°C Streptomyces cinnamoneus
3.1.1.73 1.96
-
 ethyl ferulate isozyme R43, pH 7.0, 50°C Streptomyces cinnamoneus
3.1.1.73 2.61
-
 methyl caffeate isozyme R43, pH 7.0, 50°C Streptomyces cinnamoneus
3.1.1.73 3
-
 methyl 4-coumarate isozyme R43, pH 7.0, 50°C Streptomyces cinnamoneus
3.1.1.73 3.31
-
 methyl caffeate isozyme R18, pH 7.0, 50°C Streptomyces cinnamoneus
3.1.1.73 4.28
-
 ethyl ferulate isozyme R18, pH 7.0, 50°C Streptomyces cinnamoneus
3.1.1.73 4.31
-
 methyl 4-coumarate isozyme R18, pH 7.0, 50°C Streptomyces cinnamoneus
3.1.1.73 4.41
-
 methyl ferulate isozyme R43, pH 7.0, 50°C Streptomyces cinnamoneus
3.1.1.73 4.99
-
 methyl ferulate isozyme R18, pH 7.0, 50°C Streptomyces cinnamoneus
3.1.1.73 9.4
-
 methyl sinapinate isozyme R18, pH 7.0, 50°C Streptomyces cinnamoneus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.73 extracellular the R18 sequence contains a signal sequence at the N-terminal. Although R43 has no signal peptide for secretion, the enzyme is secreted by the Streptomyces protein expression system Streptomyces cinnamoneus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 38000
-
 x * 38000, extracellular isozyme R18, SDS-PAGE, x * 52000, extracellular isozyme R43, SDS-PAGE Streptomyces cinnamoneus
3.1.1.73 52000
-
 x * 38000, extracellular isozyme R18, SDS-PAGE, x * 52000, extracellular isozyme R43, SDS-PAGE Streptomyces cinnamoneus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Streptomyces cinnamoneus
-
 isozymes R18 and R43 identified from screening of feruloyl esterases from a Streptomyces esterase library, overview
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 0.07
-
 purified recombinant isozyme R18, pH 7.0, 50°C, substrate 4-nitrophenylbutyrate Streptomyces cinnamoneus
3.1.1.73 0.37
-
 purified recombinant isozyme R43, pH 7.0, 50°C, substrate methyl vanillate Streptomyces cinnamoneus
3.1.1.73 0.49
-
 purified recombinant isozyme R43, pH 7.0, 50°C, substrate 4-nitrophenylbutyrate Streptomyces cinnamoneus
3.1.1.73 1.1
-
 purified recombinant isozyme R43, pH 7.0, 50°C, substrate methyl sinapinate Streptomyces cinnamoneus
3.1.1.73 1.54
-
 purified recombinant isozyme R43, pH 7.0, 50°C, substrate methyl caffeate Streptomyces cinnamoneus
3.1.1.73 1.89
-
 purified recombinant isozyme R18, pH 7.0, 50°C, substrate methyl vanillate Streptomyces cinnamoneus
3.1.1.73 5.4
-
 purified recombinant isozyme R18, pH 7.0, 50°C, substrate methyl caffeate Streptomyces cinnamoneus
3.1.1.73 6.75
-
 purified recombinant isozyme R43, pH 7.0, 50°C, substrate methyl 4-coumarate Streptomyces cinnamoneus
3.1.1.73 10.02
-
 purified recombinant isozyme R43, pH 7.0, 50°C, substrate ethyl ferulate Streptomyces cinnamoneus
3.1.1.73 10.9
-
 purified recombinant isozyme R18, pH 7.0, 50°C, substrate methyl sinapinate Streptomyces cinnamoneus
3.1.1.73 13.75
-
 purified recombinant isozyme R18, pH 7.0, 50°C, substrate methyl 4-coumarate Streptomyces cinnamoneus
3.1.1.73 18.97
-
 purified recombinant isozyme R18, pH 7.0, 50°C, substrate ethyl ferulate Streptomyces cinnamoneus
3.1.1.73 19.8
-
 purified recombinant isozyme R43, pH 7.0, 50°C, substrate methyl ferulate Streptomyces cinnamoneus
3.1.1.73 23.07
-
 purified recombinant isozyme R18, pH 7.0, 50°C, substrate methyl ferulate Streptomyces cinnamoneus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 4-nitrophenyl butyrate + H2O
-
 Streptomyces cinnamoneus 4-nitrophenol + butyrate
-
 ?
3.1.1.73 ethyl ferulate + H2O
-
 Streptomyces cinnamoneus ethanol + ferulate
-
 ?
3.1.1.73 methyl 4-coumarate + H2O
-
 Streptomyces cinnamoneus 4-coumarate + methanol
-
 ?
3.1.1.73 methyl caffeate + H2O
-
 Streptomyces cinnamoneus caffeic acid + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Streptomyces cinnamoneus methanol + ferulate
-
 ?
3.1.1.73 methyl sinapinate + H2O
-
 Streptomyces cinnamoneus sinapate + methanol
-
 ?
3.1.1.73 methyl vanillate + H2O
-
 Streptomyces cinnamoneus vanillate + methanol
-
 ?
3.1.1.73 additional information two isozymes from Streptomyces cinnamoneus, R18 and R43, individually show the ability to produce ferulate from corn bran. Combination with other Streptomyces enzymes, namely xylanase and alpha-L-arabinofuranosidase, increases ferulate production from biomass such as corn bran, defatted rice bran, and wheat bran Streptomyces cinnamoneus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 ? x * 38000, extracellular isozyme R18, SDS-PAGE, x * 52000, extracellular isozyme R43, SDS-PAGE Streptomyces cinnamoneus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 FAE
-
 Streptomyces cinnamoneus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 40
-
 isozyme R43 Streptomyces cinnamoneus
3.1.1.73 50
-
 isozyme R18 Streptomyces cinnamoneus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.73 15 50 activity range, isozyme R43 Streptomyces cinnamoneus
3.1.1.73 20 50 activity range, isozyme R18 Streptomyces cinnamoneus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 25 40 isozyme R43, thermolabile, 30 min, loss of 50% activity at 25°C, inactivation at 40°C Streptomyces cinnamoneus
3.1.1.73 50 60 isozyme R18, 30 min, completely stable up to 50°C, inactivation at 60°C Streptomyces cinnamoneus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 7
-
 isozyme R43 Streptomyces cinnamoneus
3.1.1.73 7.5 8 isozyme R18 Streptomyces cinnamoneus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 5 9 activity range, isozymes R18 and R43 Streptomyces cinnamoneus

General Information

EC Number General Information Comment Organism
3.1.1.73 physiological function feruloyl esterase is a key bacterial enzyme involved in ferulate production from agricultural biomass Streptomyces cinnamoneus