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Literature summary extracted from
- Novel extracellular PHB depolymerase from Streptomyces ascomycinicus: PHB copolymers degradation in acidic conditions (2013), PLoS ONE, 8, e71699.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.75 | methyl-beta-cyclodextrin | - |
Streptomyces ascomycinicus |  |
| 3.1.1.75 | PEG 3350 | - |
Streptomyces ascomycinicus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.75 | gene fkbU, recombinant expression of extracellular wild-type enzyme in Escherichia coli strain BL21(DE3) and Rhodococcus sp. strain T104, the enzyme is secreted, expression of mutants S131A, S131C, D209N, H269E and H269Q in Rhodococcus sp. T104 and secretion to the medium | Streptomyces ascomycinicus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.1.75 | D209N | site-directed mutagenesis, inactive mutant | Streptomyces ascomycinicus |
| 3.1.1.75 | H269E | site-directed mutagenesis, inactive mutant | Streptomyces ascomycinicus |
| 3.1.1.75 | H269Q | site-directed mutagenesis, inactive mutant | Streptomyces ascomycinicus |
| 3.1.1.75 | S131A | site-directed mutagenesis, inactive mutant | Streptomyces ascomycinicus |
| 3.1.1.75 | S131C | site-directed mutagenesis, inactive mutant | Streptomyces ascomycinicus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.75 | 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/glycinamide | 33% inhibition at 5 mM | Streptomyces ascomycinicus | |
| 3.1.1.75 | 2-propanol | 71% inhibition at 10% (vol/vol) | Streptomyces ascomycinicus | |
| 3.1.1.75 | acetone | 82% inhibition at 10% (vol/vol) | Streptomyces ascomycinicus | |
| 3.1.1.75 | acetonitrile | 70% inhibition at 10% (vol/vol) | Streptomyces ascomycinicus | |
| 3.1.1.75 | diethyldicarbonate | 40% inhibition at 5 mM, 90% at 20 mM | Streptomyces ascomycinicus | |
| 3.1.1.75 | diethylene glycol | 71% inhibition at 10% (vol/vol) | Streptomyces ascomycinicus | |
| 3.1.1.75 | DMF | 84% inhibition at 10% (vol/vol) | Streptomyces ascomycinicus | |
| 3.1.1.75 | DMSO | 85% inhibition at 10% (vol/vol) | Streptomyces ascomycinicus | |
| 3.1.1.75 | ethanol | 82% inhibition at 10% (vol/vol) | Streptomyces ascomycinicus | |
| 3.1.1.75 | ethylene glycol | 28% inhibition at 10% (vol/vol) | Streptomyces ascomycinicus | |
| 3.1.1.75 | methanol | 91% inhibition at 10% (vol/vol) | Streptomyces ascomycinicus | |
| 3.1.1.75 | additional information | poor inhibition by glycerol. Increase of ionic strength gradually inhibits the poly(3-hydroxybutyrate) depolymerase activity leading to its complete inactivation at 2.5 M NaCl | Streptomyces ascomycinicus | |
| 3.1.1.75 | NaCl | complete inactivation at 2.5 M | Streptomyces ascomycinicus | |
| 3.1.1.75 | PMSF | complete inactivation at 1 mM | Streptomyces ascomycinicus | |
| 3.1.1.75 | tetrahydrofuran | 51% inhibition at 10% (vol/vol) | Streptomyces ascomycinicus | |
| 3.1.1.75 | triethylene glycol | 73% inhibition at 10% (vol/vol) | Streptomyces ascomycinicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.75 | extracellular | - |
Streptomyces ascomycinicus | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.75 | Ca2+ | activates | Streptomyces ascomycinicus | |
| 3.1.1.75 | Mg2+ | activates | Streptomyces ascomycinicus | |
| 3.1.1.75 | Mn2+ | activates | Streptomyces ascomycinicus | |
| 3.1.1.75 | additional information | enzyme activity is increased in the presence of divalent cations due to non-essential activation | Streptomyces ascomycinicus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.75 | 48000 | - |
1 * 48400, about mass spectrometry, 1 * 48000, SDS-PAGE | Streptomyces ascomycinicus |
| 3.1.1.75 | 48400 | - |
1 * 48400, about mass spectrometry, 1 * 48000, SDS-PAGE | Streptomyces ascomycinicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.75 | Streptomyces ascomycinicus | Q9KIF2 | sp. nov., gene fkbU | - |
| 3.1.1.75 | Streptomyces ascomycinicus DSM Z 40822 | Q9KIF2 | sp. nov., gene fkbU | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.75 | recombinant wild-type and mutant extracellular enzyme from Escherichia coli strain BL21(DE3) and Rhodococcus sp. strain T104 by two different steps of hydrophobic interaction chromatography | Streptomyces ascomycinicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.75 | additional information | the enzyme is able to degrade polyhydroxybutyrate, but also shows ability to degrade films made of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymers and a blend of poly(3-hydroxybutyrate) and starch (7:3 proportion wt/wt) | Streptomyces ascomycinicus | ? | - |
? | |
| 3.1.1.75 | additional information | the enzyme is able to degrade polyhydroxybutyrate, but also shows ability to degrade films made of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymers and a blend of poly(3-hydroxybutyrate) and starch (7:3 proportion wt/wt) | Streptomyces ascomycinicus DSM Z 40822 | ? | - |
? | |
| 3.1.1.75 | poly(3-hydroxybutyrate) + H2O | average molecular mass of 437 kDa | Streptomyces ascomycinicus | 3-hydroxybutyrate + 3-hydroxybutyryl-3-hydroxybutyrate | - |
? | |
| 3.1.1.75 | poly(3-hydroxybutyrate) + H2O | average molecular mass of 437 kDa | Streptomyces ascomycinicus DSM Z 40822 | 3-hydroxybutyrate + 3-hydroxybutyryl-3-hydroxybutyrate | - |
? | |
| 3.1.1.75 | poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | substrate co-polymer with 5 or 12% (wt) 3-hydroxyvalerate | Streptomyces ascomycinicus | ? | - |
? | |
| 3.1.1.75 | poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | substrate co-polymer with 5 or 12% (wt) 3-hydroxyvalerate | Streptomyces ascomycinicus DSM Z 40822 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.75 | monomer | 1 * 48400, about mass spectrometry, 1 * 48000, SDS-PAGE | Streptomyces ascomycinicus |
| 3.1.1.75 | More | the enzyme has a globular shape with an alpha-beta hydrolase fold | Streptomyces ascomycinicus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.75 | PhaZSa | - |
Streptomyces ascomycinicus |
| 3.1.1.75 | PHB depolymerase | - |
Streptomyces ascomycinicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.75 | 45 | - |
- |
Streptomyces ascomycinicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.75 | 6 | - |
- |
Streptomyces ascomycinicus |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.1.1.75 | additional information | the enzyme has a globular shape with an alpha-beta hydrolase fold, three-dimensional structure and homology structure modelling, overview | Streptomyces ascomycinicus |
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Release 2023.1 (January 2023)
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