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Literature summary extracted from

  • Bou Ali, M.; Karray, A.; Gargouri, Y.; Ben Ali, Y.
    N-terminal domain of turkey pancreatic lipase is active on long chain triacylglycerols and stabilized by colipase (2013), PLoS ONE, 8, e71605.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 expressed in Pichia pastoris strain X-33 Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 diethyl 4-nitrophenyl phosphate diethyl 4-nitrophenyl phosphate is only ìnhibitory in the presence of deoxycholic acid sodium salt (2 mM) Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 35000
-
 x * 35000, SDS-PAGE Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 Ni-NTA affinity column chromatography Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 pancreas
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 3
-
 supernatant, with tributyrin as substrate, at pH 8.0 and 37°C Sus scrofa
3.1.1.3 70
-
 after 23.33fold purification, with tributyrin as substrate, at pH 8.0 and 37°C Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 olive oil + H2O
-
 Sus scrofa ?
-
 ?
3.1.1.3 tributyrin + H2O
-
 Sus scrofa ?
-
 ?
3.1.1.3 trioctanoyl glycerol + H2O
-
 Sus scrofa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 35000, SDS-PAGE Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Sus scrofa
3.1.1.3 TPL
-
 Sus scrofa
3.1.1.3 triacylglycerol acylhydrolase
-
 Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 37
-
 the enzyme is unstable at temperatures over 37°C with less than 40% activity at 40°C, about 25% activity at 45°C, and no activity at 50°C, after 30 min of incubation Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 4 6 the enzyme loses 70% of its activity at acid pH, after 5 min of incubation Sus scrofa