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Literature summary extracted from

  • Puri, R.V.; Singh, N.; Gupta, R.K.; Tyagi, A.K.
    Endonuclease IV Is the major apurinic/apyrimidinic endonuclease in Mycobacterium tuberculosis and is important for protection against oxidative damage (2013), PLoS ONE, 8, e71535.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.21.2 expressed in Escherichia coli BL21(lambdaDE3) cells Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.2 dithiothreitol complete inhibition at 50 mM Mycobacterium tuberculosis
3.1.21.2 EDTA complete inhibition at 50 mM Mycobacterium tuberculosis
3.1.21.2 additional information the enzyme is tolerant to EDTA up to 2 mM Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.21.2 0.00000691
-
5'-end-labeled 29 nucleotide double-stranded DNA at pH 8.0 and 37°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.2 Ca2+ stimulates activity Mycobacterium tuberculosis
3.1.21.2 Mg2+ maximally stimulated in the presence of Mg2+ Mycobacterium tuberculosis
3.1.21.2 Mn2+ slight stimulation Mycobacterium tuberculosis
3.1.21.2 additional information not stimulated by Zn2+, Co2+ Mycobacterium tuberculosis
3.1.21.2 additional information the enzyme shows metal ion independent 3'->5' exonuclease activity Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.21.2 26800
-
x * 26800, SDS-PAGE Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.2 Mycobacterium tuberculosis P9WQ13
-
-
3.1.21.2 Mycobacterium tuberculosis H37Rv P9WQ13
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.2 Streptactin column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.2 5'-end-labeled 19 nucleotide single-stranded DNA + H2O
-
Mycobacterium tuberculosis ?
-
?
3.1.21.2 5'-end-labeled 19 nucleotide single-stranded DNA + H2O
-
Mycobacterium tuberculosis H37Rv ?
-
?
3.1.21.2 5'-end-labeled 29 nucleotide double-stranded DNA + H2O 92% cleavage after 30 min Mycobacterium tuberculosis ?
-
?
3.1.21.2 5'-end-labeled 29 nucleotide double-stranded DNA + H2O 92% cleavage after 30 min Mycobacterium tuberculosis H37Rv ?
-
?
3.1.21.2 additional information endonuclease activity of the enzyme is about 2fold higher for double-stranded DNA in comparison to single-stranded DNA Mycobacterium tuberculosis ?
-
?
3.1.21.2 additional information the enzyme also shows 3'->5' exonuclease activity Mycobacterium tuberculosis ?
-
?
3.1.21.2 additional information endonuclease activity of the enzyme is about 2fold higher for double-stranded DNA in comparison to single-stranded DNA Mycobacterium tuberculosis H37Rv ?
-
?
3.1.21.2 additional information the enzyme also shows 3'->5' exonuclease activity Mycobacterium tuberculosis H37Rv ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.21.2 ? x * 26800, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
3.1.21.2 End
-
Mycobacterium tuberculosis
3.1.21.2 endonuclease IV
-
Mycobacterium tuberculosis
3.1.21.2 Rv0670
-
Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.21.2 0.0193
-
5'-end-labeled 29 nucleotide double-stranded DNA at pH 8.0 and 37°C Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
3.1.21.2 physiological function the enzyme plays a crucial role in defense against oxidative stress Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.21.2 2790
-
5'-end-labeled 29 nucleotide double-stranded DNA at pH 8.0 and 37°C Mycobacterium tuberculosis