EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.21.2 | expressed in Escherichia coli BL21(lambdaDE3) cells | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.21.2 | dithiothreitol | complete inhibition at 50 mM | Mycobacterium tuberculosis | |
3.1.21.2 | EDTA | complete inhibition at 50 mM | Mycobacterium tuberculosis | |
3.1.21.2 | additional information | the enzyme is tolerant to EDTA up to 2 mM | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.21.2 | 0.00000691 | - |
5'-end-labeled 29 nucleotide double-stranded DNA | at pH 8.0 and 37°C | Mycobacterium tuberculosis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.21.2 | Ca2+ | stimulates activity | Mycobacterium tuberculosis | |
3.1.21.2 | Mg2+ | maximally stimulated in the presence of Mg2+ | Mycobacterium tuberculosis | |
3.1.21.2 | Mn2+ | slight stimulation | Mycobacterium tuberculosis | |
3.1.21.2 | additional information | not stimulated by Zn2+, Co2+ | Mycobacterium tuberculosis | |
3.1.21.2 | additional information | the enzyme shows metal ion independent 3'->5' exonuclease activity | Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.21.2 | 26800 | - |
x * 26800, SDS-PAGE | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.21.2 | Mycobacterium tuberculosis | P9WQ13 | - |
- |
3.1.21.2 | Mycobacterium tuberculosis H37Rv | P9WQ13 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.21.2 | Streptactin column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.21.2 | 5'-end-labeled 19 nucleotide single-stranded DNA + H2O | - |
Mycobacterium tuberculosis | ? | - |
? | |
3.1.21.2 | 5'-end-labeled 19 nucleotide single-stranded DNA + H2O | - |
Mycobacterium tuberculosis H37Rv | ? | - |
? | |
3.1.21.2 | 5'-end-labeled 29 nucleotide double-stranded DNA + H2O | 92% cleavage after 30 min | Mycobacterium tuberculosis | ? | - |
? | |
3.1.21.2 | 5'-end-labeled 29 nucleotide double-stranded DNA + H2O | 92% cleavage after 30 min | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
3.1.21.2 | additional information | endonuclease activity of the enzyme is about 2fold higher for double-stranded DNA in comparison to single-stranded DNA | Mycobacterium tuberculosis | ? | - |
? | |
3.1.21.2 | additional information | the enzyme also shows 3'->5' exonuclease activity | Mycobacterium tuberculosis | ? | - |
? | |
3.1.21.2 | additional information | endonuclease activity of the enzyme is about 2fold higher for double-stranded DNA in comparison to single-stranded DNA | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
3.1.21.2 | additional information | the enzyme also shows 3'->5' exonuclease activity | Mycobacterium tuberculosis H37Rv | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.21.2 | ? | x * 26800, SDS-PAGE | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.21.2 | End | - |
Mycobacterium tuberculosis |
3.1.21.2 | endonuclease IV | - |
Mycobacterium tuberculosis |
3.1.21.2 | Rv0670 | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.21.2 | 0.0193 | - |
5'-end-labeled 29 nucleotide double-stranded DNA | at pH 8.0 and 37°C | Mycobacterium tuberculosis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.21.2 | physiological function | the enzyme plays a crucial role in defense against oxidative stress | Mycobacterium tuberculosis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.21.2 | 2790 | - |
5'-end-labeled 29 nucleotide double-stranded DNA | at pH 8.0 and 37°C | Mycobacterium tuberculosis |