Literature summary extracted from

Jacewicz, A.; Izumi, A.; Brunner, K.; Schnell, R.; Schneider, G.
Structural insights into the UbiD protein family from the crystal structure of PA0254 from Pseudomonas aeruginosa (2013), PLoS ONE, 8, e63161.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
4.1.1.98	expressed in Escherichia coli BL21(DE3) cells	Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
4.1.1.98	sitting drop vapor diffusion method, using 1 M (NH4)2SO4, 0.1 M Bis-Tris, pH 5.5, 1% (w/v) PEG 3350 or 0.1 M HEPES, pH 7.5, 0.2 M MgCl2, 10% (w/v) PEG 400	Pseudomonas aeruginosa

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
4.1.1.98	Mg2+	contains a Mg2+ binding site	Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
4.1.1.98	57614	-	2 * 57614, SeMet-substituted protein, calculated from amino acid sequence	Pseudomonas aeruginosa

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.1.1.98	Pseudomonas aeruginosa	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
4.1.1.98	Ni-NTA agarose bead chromatography and Sephacryl S200 gel filtration	Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.1.1.98	3-octaprenyl-4-hydroxybenzoate	-	Pseudomonas aeruginosa	2-octaprenylphenol + CO2	-	?

Subunits

EC Number	Subunits	Comment	Organism
4.1.1.98	homodimer	2 * 57614, SeMet-substituted protein, calculated from amino acid sequence	Pseudomonas aeruginosa

Synonyms

EC Number	Synonyms	Comment	Organism
4.1.1.98	PA0254	-	Pseudomonas aeruginosa
4.1.1.98	ubiD	-	Pseudomonas aeruginosa

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
4.1.1.98	additional information	the enzyme does not bind FMN	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)