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Literature summary extracted from

  • van Vugt-Lussenburg, B.M.; van der Weel, L.; Hagen, W.R.; Hagedoorn, P.L.
    Biochemical similarities and differences between the catalytic [4Fe-4S] cluster containing fumarases FumA and FumB from Escherichia coli (2013), PLoS ONE, 8, e55549.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.2 expressed in Escherichia coli Escherichia coli

General Stability

EC Number General Stability Organism
4.2.1.2 exposure to air results in 30% decreased activity Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.2 0.3
-
 L-malate pH 8, 37°C, Vmax: 490 micromol/minute/mg/ Escherichia coli
4.2.1.2 0.32
-
 fumarate pH 8, 37°C, Vmax: 1430 micromol/minute/mg/ Escherichia coli
4.2.1.2 0.46
-
 fumarate pH 8, 37°C, Vmax: 1900 micromol/minute/mg/ Escherichia coli
4.2.1.2 0.7
-
 D-Tartrate pH 8, 37°C, Vmax: 2.3 micromol/minute/mg/ Escherichia coli
4.2.1.2 0.7
-
 L-malate pH 8, 37°C, Vmax: 720 micromol/minute/mg/ Escherichia coli
4.2.1.2 0.8
-
 D-Tartrate pH 8, 37°C, Vmax: 9.2 micromol/minute/mg/ Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.2 [3Fe-4S] center 10-25% of purified protein contains [3Fe-4S] cluster Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.2 120000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.2 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.2 D-tartrate
-
 Escherichia coli ?
-
 ?
4.2.1.2 L-malate
-
 Escherichia coli fumarate + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.2 homodimer
-
 Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
4.2.1.2 FumA
-
 Escherichia coli
4.2.1.2 FumB
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.2 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.2 8
-
 assay at Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.2 3
-
 D-Tartrate pH 8, 37°C Escherichia coli
4.2.1.2 12
-
 D-Tartrate pH 8, 37°C Escherichia coli
4.2.1.2 1000
-
 L-malate pH 8, 37°C/ Escherichia coli
4.2.1.2 1600
-
 L-malate pH 8, 37°C Escherichia coli
4.2.1.2 4200
-
 fumarate pH 8, 37°C Escherichia coli
4.2.1.2 4500
-
 fumarate pH 8, 37°C Escherichia coli