EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.B41 | - |
Archaeoglobus fulgidus |
2.3.1.B43 | - |
Homo sapiens |
2.3.1.286 | - |
Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.B41 | M70R | further decrease in nicotinamide sensitivity compared to wild-type | Archaeoglobus fulgidus |
2.3.1.B43 | R105L | mutant retains wild-type deacetylation activity and partially gains Sirt3-like nicotinamide sensitivity (IC50: 0.189 mM) | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.B41 | additional information | nicotinamide insensitivity, IC50: 2.1 mM | Archaeoglobus fulgidus | |
2.3.1.B43 | nicotinamide | nicotinamide inhibition of Sirt5-dependent deacetylation features weak substrate-dependence but requires high, nonphysiological nicotinamide concentrations. The insensitivity of Sirt5 to physiological nicotinamide concentrations is due to a lack of non-competitive inhibition via base exchange, and the inhibition observed at very high nicotinamide concentrations is likely caused by competition with NAD+ | Homo sapiens | |
2.3.1.286 | nicotinamide | 0.5 mM, complete inhibition | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.B41 | 0.22 | - |
SKEYFS(N6-acetyl)KQK | pH 7.8, 37°C | Archaeoglobus fulgidus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.B41 | Archaeoglobus fulgidus | O30124 | - |
- |
2.3.1.B43 | Homo sapiens | Q9NXA8 | - |
- |
2.3.1.286 | Homo sapiens | Q9NTG7 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.B41 | - |
Archaeoglobus fulgidus |
2.3.1.B43 | - |
Homo sapiens |
2.3.1.286 | - |
Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.B41 | additional information | insignificant activity with SKEYFS(N6-succinyl)KQK | Archaeoglobus fulgidus | ? | - |
? | |
2.3.1.B41 | NAD+ + SKEYFS(N6-acetyl)KQK | - |
Archaeoglobus fulgidus | nicotinamide + SKEYFSKQK + 2'-O-acetyl-ADP-ribose | - |
? | |
2.3.1.B43 | NAD+ + benzoyl-RGVL(N6-succinyl)KEYGV-amide | carbamoyl phosphate synthetase 1 Lys527 peptide | Homo sapiens | nicotinamide + benzoyl-RGVLKEYGV-amide + 2'-O-acetyl-ADP-ribose | - |
? | |
2.3.1.B43 | NAD+ + FKRGVL(N6-acetyl)KEYGVKV | carbamoyl phosphate synthetase 1 Lys527 peptide | Homo sapiens | nicotinamide + FKRGVLKEYGVKV + 2'-O-acetyl-ADP-ribose | - |
? | |
2.3.1.B43 | NAD+ + TRSG(N6-acetyl)KVMR | peptide based on an acetyl-CoA synthetase 2 acetylation site | Homo sapiens | nicotinamide + TRSGKVMR + 2'-O-acetyl-ADP-ribose | - |
? | |
2.3.1.286 | NAD+ + TRSG(N6-acetyl)KVMR | peptide based on an acetyl-CoA synthetase 2 acetylation site | Homo sapiens | nicotinamide + TRSGKVMR + 2'-O-acetyl-ADP-ribose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.B41 | Sir2Af2 | - |
Archaeoglobus fulgidus |
2.3.1.B43 | SIRT5 | - |
Homo sapiens |
2.3.1.286 | SIRT3 | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.B41 | 37 | - |
assay at | Archaeoglobus fulgidus |
2.3.1.B43 | 37 | - |
assay at | Homo sapiens |
2.3.1.286 | 37 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.B41 | 7.8 | - |
assay at | Archaeoglobus fulgidus |
2.3.1.B43 | 7.8 | - |
assay at | Homo sapiens |
2.3.1.286 | 7.8 | - |
assay at | Homo sapiens |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.3.1.B43 | 0.021 | - |
pH 7.8, 37°C, substrate: benzoyl-RGVL(succK)EYGV-amide | Homo sapiens | nicotinamide | |
2.3.1.B43 | 0.7 | - |
pH 7.8, 37°C, substrate: TRSG(acK)VMR | Homo sapiens | nicotinamide | |
2.3.1.B43 | 1.6 | - |
pH 7.8, 37°C, substrate: FKRGVL(acK)EYGVKV | Homo sapiens | nicotinamide | |
2.3.1.286 | 0.043 | - |
pH 7.8, 37°C, substrate: TRSG(N6-acetyl)KVMR | Homo sapiens | nicotinamide |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.B41 | 0.132 | - |
SKEYFS(N6-acetyl)KQK | pH 7.8, 37°C | Archaeoglobus fulgidus |