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Literature summary extracted from

  • Fischer, F.; Gertz, M.; Suenkel, B.; Lakshminarasimhan, M.; Schutkowski, M.; Steegborn, C.
    Sirt5 deacylation activities show differential sensitivities to nicotinamide inhibition (2012), PLoS One, 7, e45098.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.B41
-
Archaeoglobus fulgidus
2.3.1.B43
-
Homo sapiens
2.3.1.286
-
Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.B41 M70R further decrease in nicotinamide sensitivity compared to wild-type Archaeoglobus fulgidus
2.3.1.B43 R105L mutant retains wild-type deacetylation activity and partially gains Sirt3-like nicotinamide sensitivity (IC50: 0.189 mM) Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.B41 additional information nicotinamide insensitivity, IC50: 2.1 mM Archaeoglobus fulgidus
2.3.1.B43 nicotinamide nicotinamide inhibition of Sirt5-dependent deacetylation features weak substrate-dependence but requires high, nonphysiological nicotinamide concentrations. The insensitivity of Sirt5 to physiological nicotinamide concentrations is due to a lack of non-competitive inhibition via base exchange, and the inhibition observed at very high nicotinamide concentrations is likely caused by competition with NAD+ Homo sapiens
2.3.1.286 nicotinamide 0.5 mM, complete inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.B41 0.22
-
SKEYFS(N6-acetyl)KQK pH 7.8, 37°C Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.B41 Archaeoglobus fulgidus O30124
-
-
2.3.1.B43 Homo sapiens Q9NXA8
-
-
2.3.1.286 Homo sapiens Q9NTG7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.B41
-
Archaeoglobus fulgidus
2.3.1.B43
-
Homo sapiens
2.3.1.286
-
Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.B41 additional information insignificant activity with SKEYFS(N6-succinyl)KQK Archaeoglobus fulgidus ?
-
?
2.3.1.B41 NAD+ + SKEYFS(N6-acetyl)KQK
-
Archaeoglobus fulgidus nicotinamide + SKEYFSKQK + 2'-O-acetyl-ADP-ribose
-
?
2.3.1.B43 NAD+ + benzoyl-RGVL(N6-succinyl)KEYGV-amide carbamoyl phosphate synthetase 1 Lys527 peptide Homo sapiens nicotinamide + benzoyl-RGVLKEYGV-amide + 2'-O-acetyl-ADP-ribose
-
?
2.3.1.B43 NAD+ + FKRGVL(N6-acetyl)KEYGVKV carbamoyl phosphate synthetase 1 Lys527 peptide Homo sapiens nicotinamide + FKRGVLKEYGVKV + 2'-O-acetyl-ADP-ribose
-
?
2.3.1.B43 NAD+ + TRSG(N6-acetyl)KVMR peptide based on an acetyl-CoA synthetase 2 acetylation site Homo sapiens nicotinamide + TRSGKVMR + 2'-O-acetyl-ADP-ribose
-
?
2.3.1.286 NAD+ + TRSG(N6-acetyl)KVMR peptide based on an acetyl-CoA synthetase 2 acetylation site Homo sapiens nicotinamide + TRSGKVMR + 2'-O-acetyl-ADP-ribose
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.B41 Sir2Af2
-
Archaeoglobus fulgidus
2.3.1.B43 SIRT5
-
Homo sapiens
2.3.1.286 SIRT3
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.B41 37
-
assay at Archaeoglobus fulgidus
2.3.1.B43 37
-
assay at Homo sapiens
2.3.1.286 37
-
assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.B41 7.8
-
assay at Archaeoglobus fulgidus
2.3.1.B43 7.8
-
assay at Homo sapiens
2.3.1.286 7.8
-
assay at Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.B43 0.021
-
pH 7.8, 37°C, substrate: benzoyl-RGVL(succK)EYGV-amide Homo sapiens nicotinamide
2.3.1.B43 0.7
-
pH 7.8, 37°C, substrate: TRSG(acK)VMR Homo sapiens nicotinamide
2.3.1.B43 1.6
-
pH 7.8, 37°C, substrate: FKRGVL(acK)EYGVKV Homo sapiens nicotinamide
2.3.1.286 0.043
-
pH 7.8, 37°C, substrate: TRSG(N6-acetyl)KVMR Homo sapiens nicotinamide

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.B41 0.132
-
SKEYFS(N6-acetyl)KQK pH 7.8, 37°C Archaeoglobus fulgidus