Literature summary extracted from

Oliver, J.D.; Kaye, S.J.; Tuckwell, D.; Johns, A.E.; Macdonald, D.A.; Livermore, J.; Warn, P.A.; Birch, M.; Bromley, M.J.
The Aspergillus fumigatus dihydroxyacid dehydratase Ilv3A/IlvC is required for full virulence (2012), PLoS ONE, 7, e43559.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
4.2.1.9	recombinant expression in Escherichia coli as a His-tagged fusion protein	Aspergillus fumigatus

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
4.2.1.9	2-hydroxy-3-methylbutyric acid	-	Aspergillus fumigatus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.2.1.9	10.1	-	L-threonate	pH 8, 22°C	Aspergillus fumigatus

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
4.2.1.9	mitochondrion	predicted localisation	Aspergillus fumigatus	5739	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
4.2.1.9	65000	-	SDS-PAGE, recombinant enzyme	Aspergillus fumigatus

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.2.1.9	Aspergillus fumigatus	-	-	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
4.2.1.9	18	-	pH 8, 22°C	Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.2.1.9	L-threonate	-	Aspergillus fumigatus	?	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
4.2.1.9	AfIlv3A	-	Aspergillus fumigatus
4.2.1.9	AFUA_2G2410	-	Aspergillus fumigatus
4.2.1.9	DHAD	-	Aspergillus fumigatus
4.2.1.9	dihydroxyacid dehydratase	-	Aspergillus fumigatus
4.2.1.9	ilvC	-	Aspergillus fumigatus

IC50 Value

EC Number	IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
4.2.1.9	8	-	pH 8, 22°C	Aspergillus fumigatus	2-hydroxy-3-methylbutyric acid

General Information

EC Number	General Information	Comment	Organism
4.2.1.9	malfunction	deletion Dilv3A strain requires supplementation with isoleucine and valine for growth	Aspergillus fumigatus
4.2.1.9	malfunction	mutants lacking AfIlv3A and ilv3B exhibit reduced levels of virulence in murine infection models	Aspergillus fumigatus

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Release 2023.1 (January 2023)