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Literature summary extracted from

  • Johansson, A.L.; Collins, R.; Arner, E.S.; Brzezinski, P.; Hoegbom, M.
    Biochemical discrimination between selenium and sulfur 2: mechanistic investigation of the selenium specificity of human selenocysteine lyase (2012), PLoS ONE, 7, e30528.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.16 recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.4.1.16 D146K site-directed mutagenesis, the mutant variant shows loss of selenocysteine specificity and appearance of cysteine desulfurase activity Homo sapiens
4.4.1.16 D146K/H389T site-directed mutagenesis,the mutant variant shows loss of selenocysteine specificity and appearance of cysteine desulfurase activity to a higher extent Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.16 L-selenocysteine + reduced acceptor Homo sapiens
-
 selenide + L-alanine + acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.16 Homo sapiens Q96I15
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.16 recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration, followed by tag cleavage through TEV protease, another step of nickel affinity chromatography, and dialysis Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
4.4.1.16 L-selenocysteine + reduced acceptor = selenide + L-alanine + acceptor reaction mechanism and mechanism of substrate recognition, model, overview. A pyridoxal 5'-phosphate-substrate intermediate is attacked by a deprotonated active site Cys, producing the protein-bound persulfide or sulfoselenide, formation of the external Cys-aldimine intermediate. Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.16 L-selenocysteine + reduced acceptor
-
 Homo sapiens selenide + L-alanine + acceptor
-
 ?
4.4.1.16 additional information the enzyme is specific for L-selenocysteine versus L-cysteine, substrate specificity for selenium and sulfur is determined by a single residue, Asp246, in human selenocysteine lyase, chemical basis for and mechanism of the enzyme's substrate discrimination of wild-type enzyme and mutant D146K/H389T, overview. The wild-type reaction with Cys is a single-turnover reaction, producing a dead-end protein adduct Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.16 SCL
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.16 pyridoxal 5'-phosphate
-
 Homo sapiens