BRENDA - Enzyme Database show

Inhibition of 5-aminolevulinic acid dehydratase by mercury in excised greening maize leaf segments

Gupta, P.; Jain, M.; Sarangthem, J.; Gadre, R.; Plant Physiol. Biochem. 62, 63-69 (2013)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
5,5'-dithio(bis-2-nitrobenzoic acid)
-
Zea mays
4.2.1.24
Hg2+
the inhibition caused by 0.37 mM Hg2+ is alleviated by addition of 10 mM KNO3. 10 mM NH4Cl and 5 mM sucrose increase the inhibitory effect of Hg2+ on enzyme activity, while 10 mM levulinic acid and 0.0001 mM 5,5'-dithio(bis-2-nitrobenzoic acid) glutamine and glutathione decrease it
Zea mays
4.2.1.24
levulinic acid
competitive inhibitor, 8% residual activity at 20 mM
Zea mays
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.347
-
5-aminolevulinate
in the presence of Hg2+, at pH 8.2 and 37C
Zea mays
4.2.1.24
0.532
-
5-aminolevulinate
in the absence of Hg2+, at pH 8.2 and 37C
Zea mays
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.24
5-aminolevulinate
Zea mays
-
porphobilinogen + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.24
Zea mays
-
cultivar GSF-2
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.24
leaf
-
Zea mays
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate
-
730630
Zea mays
porphobilinogen + H2O
-
-
-
?
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.24
5,5'-dithio(bis-2-nitrobenzoic acid)
-
Zea mays
4.2.1.24
Hg2+
the inhibition caused by 0.37 mM Hg2+ is alleviated by addition of 10 mM KNO3. 10 mM NH4Cl and 5 mM sucrose increase the inhibitory effect of Hg2+ on enzyme activity, while 10 mM levulinic acid and 0.0001 mM 5,5'-dithio(bis-2-nitrobenzoic acid) glutamine and glutathione decrease it
Zea mays
4.2.1.24
levulinic acid
competitive inhibitor, 8% residual activity at 20 mM
Zea mays
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.24
0.347
-
5-aminolevulinate
in the presence of Hg2+, at pH 8.2 and 37C
Zea mays
4.2.1.24
0.532
-
5-aminolevulinate
in the absence of Hg2+, at pH 8.2 and 37C
Zea mays
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.24
5-aminolevulinate
Zea mays
-
porphobilinogen + H2O
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.24
leaf
-
Zea mays
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.24
5-aminolevulinate
-
730630
Zea mays
porphobilinogen + H2O
-
-
-
?