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Literature summary extracted from

  • Huang, S.; Zhang, J.; Ma, Y.; Wei, S.; Huang, L.
    Characterization of an acid phosphatase responsible for hydrolysis of pyridoxal 5-phosphate in tobacco plants (2012), Plant Physiol. Biochem., 57, 114-119.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 4-nitrophenyl phosphate competitive Nicotiana tabacum
3.1.3.2 ATP 1 mM, 29% residual activity, competitive Nicotiana tabacum
3.1.3.2 EDTA 1 mM, 62% residual activity Nicotiana tabacum
3.1.3.2 fluoride 1 mM, 56% residual activity Nicotiana tabacum
3.1.3.2 molybdate 1 mM, 5% residual activity Nicotiana tabacum
3.1.3.2 additional information not inhibitory: levamisole and tartrate Nicotiana tabacum
3.1.3.2 phosphate 1 mM, 20% residual activity, competitive Nicotiana tabacum
3.1.3.2 pyridoxal 1 mM, 20% residual activity, competitive Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.24
-
 pyridoxal 5'-phosphate pH 5.5, 37°C Nicotiana tabacum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 Ca2+ 1 mM, 179% of initial activity Nicotiana tabacum
3.1.3.2 Mg2+ most effective dimetal ion for catalysis under saturating substrate concentrations, 1 mM, 215% of initial activity Nicotiana tabacum
3.1.3.2 Mn2+ 1 mM, 154% of initial activity Nicotiana tabacum
3.1.3.2 additional information a divalent metal ion is required for activity Nicotiana tabacum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 24900
-
 2 * 24900, SDS-PAGE Nicotiana tabacum
3.1.3.2 50000
-
 gel filtration Nicotiana tabacum

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Nicotiana tabacum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2
-
 Nicotiana tabacum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 leaf
-
 Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 1998
-
 pH 5.5, 37°C Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 4-nitrophenyl phosphate + H2O
-
 Nicotiana tabacum 4-nitrophenol + phosphate about 158% of the activity with pyridoxal 5'-phosphate ?
3.1.3.2 diphosphate + H2O
-
 Nicotiana tabacum 2 phosphate about 156% of the activity with pyridoxal 5'-phosphate ?
3.1.3.2 pyridoxal 5'-phosphate + H2O
-
 Nicotiana tabacum pyridoxal + phosphate
-
 ?
3.1.3.2 pyridoxamine 5'-phosphate + H2O
-
 Nicotiana tabacum pyridoxamine + phosphate about 50% of the activity with pyridoxal 5'-phosphate ?
3.1.3.2 pyridoxine 5'-phosphate + H2O
-
 Nicotiana tabacum pyridoxine + phosphate about 50% of the activity with pyridoxal 5'-phosphate ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 dimer 2 * 24900, SDS-PAGE Nicotiana tabacum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 50
-
-
 Nicotiana tabacum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 37
-
 citric acid-sodium citrate buffer, pH 5.5, fully stable at least for 120 min Nicotiana tabacum
3.1.3.2 50
-
 half-life around 50 min Nicotiana tabacum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5.5
-
-
 Nicotiana tabacum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.2 5 6 37°C for 2 h, more than 80% of total activities Nicotiana tabacum