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Literature summary extracted from

  • Mustafiz, A.; Ghosh, A.; Tripathi, A.K.; Kaur, C.; Ganguly, A.K.; Bhavesh, N.S.; Tripathi, J.K.; Pareek, A.; Sopory, S.K.; Singla-Pareek, S.L.
    A unique Ni2+ -dependent and methylglyoxal-inducible rice glyoxalase I possesses a single active site and functions in abiotic stress response (2014), Plant J., 78, 951-963.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.5 gene GLX-I or LOC_Os08g09250, functional complementation of Saccaromyces cerevisiae GLY I mutant DELTAGLO I, heterologous expression in Escherichia coli and Nicotiana tabacum resulting in improved adaptation to various abiotic stresses caused by increased scavenging of methylglyoxal, lower Na+/K+ ratio and maintenance of reduced glutathione levels in the hosts Oryza sativa Japonica Group

Protein Variants

EC Number Protein Variants Comment Organism
4.4.1.5 E145Q the mutant shows altered thermodynamic parameters for Ni2+ ion binding compared to the wild-type enzyme Oryza sativa Japonica Group
4.4.1.5 E209D inactive mutant Oryza sativa Japonica Group
4.4.1.5 E78Q the mutant shows altered thermodynamic parameters for Ni2+ ion binding compared to the wild-type enzyme Oryza sativa Japonica Group

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.5 EDTA strong inhibition Oryza sativa Japonica Group

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.5 0.0998
-
 (R)-S-lactoylglutathione recombinant enzyme, pH 7.2, temperature not specified in the publication Oryza sativa Japonica Group

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.4.1.5 Co2+ results in about 25% of the activity with Ni2+ Oryza sativa Japonica Group
4.4.1.5 Mg2+ only slight activation Oryza sativa Japonica Group
4.4.1.5 additional information no activating effect by Zn2+, Ca2+, Fe2+, and Mn2+ Oryza sativa Japonica Group
4.4.1.5 Ni2+ dependent on, required for activity, Glu145 and Glu209 are involved in Ni2+ binding, Glu78 is not involved, the monomeric enzyme possesses a single Ni2+ coordination site despite containing two GLY I domains, 0.052 mol/mol enzyme for the recombinant enzyme expressed in Escherichia coli, and 1.258 mol/mol enzyme after exogenous addition of Ni2+ Oryza sativa Japonica Group

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.5 34900
-
 recombinant enzyme, gel filtration Oryza sativa Japonica Group
4.4.1.5 36000
-
 1 * 36000, recombinant enzyme, SDS-PAGE Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.5 glutathione + methylglyoxal Oryza sativa Japonica Group
-
 (R)-S-lactoylglutathione
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.5 Oryza sativa Japonica Group Q948T6 subsp. japonica, gene GLX-I or LOC_Os08g09250
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.5 leaf
-
 Oryza sativa Japonica Group
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.5 8.3
-
 purified recombinant enzyme without metal ion, pH 7.2, temperature not specified in the publication Oryza sativa Japonica Group
4.4.1.5 120
-
 purified recombinant enzyme with Ni2+ addition, pH 7.2, temperature not specified in the publication Oryza sativa Japonica Group

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.5 (R)-S-lactoylglutathione
-
 Oryza sativa Japonica Group glutathione + methylglyoxal
-
 r
4.4.1.5 glutathione + methylglyoxal
-
 Oryza sativa Japonica Group (R)-S-lactoylglutathione
-
 r

Subunits

EC Number Subunits Comment Organism
4.4.1.5 monomer 1 * 36000, recombinant enzyme, SDS-PAGE Oryza sativa Japonica Group
4.4.1.5 More homology-based structural modeling, overview Oryza sativa Japonica Group

Synonyms

EC Number Synonyms Comment Organism
4.4.1.5 Gly I
-
 Oryza sativa Japonica Group
4.4.1.5 glyoxalase I
-
 Oryza sativa Japonica Group
4.4.1.5 OsGLYI-11.2
-
 Oryza sativa Japonica Group

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.5 70.96
-
 (R)-S-lactoylglutathione recombinant enzyme, pH 7.2, temperature not specified in the publication Oryza sativa Japonica Group

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.5 7.2 7.3
-
 Oryza sativa Japonica Group

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.4.1.5 5.5 8 high activity at, recombinant enzyme Oryza sativa Japonica Group

Expression

EC Number Organism Comment Expression
4.4.1.5 Oryza sativa Japonica Group the enzyme is highly substrate-inducible up

General Information

EC Number General Information Comment Organism
4.4.1.5 metabolism the glyoxalase system constitutes the major pathway for the detoxification of metabolically produced cytotoxin methylglyoxal into a non-toxic metabolite D-lactate Oryza sativa Japonica Group
4.4.1.5 additional information homology-based structural modeling, overview Oryza sativa Japonica Group

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.4.1.5 7100
-
 (R)-S-lactoylglutathione recombinant enzyme, pH 7.2, temperature not specified in the publication Oryza sativa Japonica Group