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Literature summary extracted from

  • Shen, G.M.; Wang, X.N.; Dou, W.; Wang, J.J.
    Biochemical and molecular characterisation of acetylcholinesterase in four field populations of Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) (2012), Pest Manag. Sci., 68, 1553-1563.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.7
-
 Bactrocera dorsalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.7 Aldicarb
-
 Bactrocera dorsalis
3.1.1.7 BW284c51
-
 Bactrocera dorsalis
3.1.1.7 eserine strongest inhibition Bactrocera dorsalis
3.1.1.7 paraoxon-ethyl
-
 Bactrocera dorsalis
3.1.1.7 propoxur
-
 Bactrocera dorsalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.7 0.0877
-
 acetylthiocholine enzyme from larva, at pH 7.4 and 37°C Bactrocera dorsalis
3.1.1.7 0.3817
-
 acetylthiocholine enzyme from pupa, at pH 7.4 and 37°C Bactrocera dorsalis
3.1.1.7 0.6993
-
 acetylthiocholine enzyme from adult, at pH 7.4 and 37°C Bactrocera dorsalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.7 55000
-
 x * 55000, SDS-PAGE Bactrocera dorsalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.7 acetylcholine + H2O Bactrocera dorsalis
-
 choline + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.7 Bactrocera dorsalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.7 procainamide affinity column chromatography Bactrocera dorsalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.7 adult
-
 Bactrocera dorsalis
-
3.1.1.7 larva
-
 Bactrocera dorsalis
-
3.1.1.7 pupa
-
 Bactrocera dorsalis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.7 0.00412
-
 crude enzyme from adult, at pH 7.4 and 37°C Bactrocera dorsalis
3.1.1.7 0.00423
-
 crude enzyme from pupa, at pH 7.4 and 37°C Bactrocera dorsalis
3.1.1.7 0.00685
-
 crude enzyme from larva, at pH 7.4 and 37°C Bactrocera dorsalis
3.1.1.7 2.082
-
 505fold purified enzyme from adult, at pH 7.4 and 37°C Bactrocera dorsalis
3.1.1.7 2.24
-
 530fold purified enzyme from pupa, at pH 7.4 and 37°C Bactrocera dorsalis
3.1.1.7 3.998
-
 584fold purified enzyme from larva, at pH 7.4 and 37°C Bactrocera dorsalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.7 acetylcholine + H2O
-
 Bactrocera dorsalis choline + acetate
-
 ?
3.1.1.7 acetylthiocholine + H2O
-
 Bactrocera dorsalis thiocholine + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.7 ? x * 55000, SDS-PAGE Bactrocera dorsalis

Synonyms

EC Number Synonyms Comment Organism
3.1.1.7 AChE
-
 Bactrocera dorsalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.7 37
-
-
 Bactrocera dorsalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.7 587.1
-
 acetylthiocholine enzyme from adult, at pH 7.4 and 37°C Bactrocera dorsalis
3.1.1.7 1079
-
 acetylthiocholine enzyme from pupa, at pH 7.4 and 37°C Bactrocera dorsalis
3.1.1.7 1820
-
 acetylthiocholine enzyme from larva, at pH 7.4 and 37°C Bactrocera dorsalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.7 7.4
-
-
 Bactrocera dorsalis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.7 Bactrocera dorsalis calculated from amino acid sequence
-
 6.4

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.1.7 0.00000042
-
 enzyme from larva, at pH 7.4 and 37°C Bactrocera dorsalis eserine
3.1.1.7 0.00000158
-
 enzyme from pupa, at pH 7.4 and 37°C Bactrocera dorsalis eserine
3.1.1.7 0.00000256
-
 enzyme from adult, at pH 7.4 and 37°C Bactrocera dorsalis eserine
3.1.1.7 0.0000071
-
 enzyme from larva, at pH 7.4 and 37°C Bactrocera dorsalis paraoxon-ethyl
3.1.1.7 0.00001782
-
 enzyme from pupa, at pH 7.4 and 37°C Bactrocera dorsalis paraoxon-ethyl
3.1.1.7 0.00001906
-
 enzyme from larva, at pH 7.4 and 37°C Bactrocera dorsalis BW284c51
3.1.1.7 0.00002304
-
 enzyme from larva, at pH 7.4 and 37°C Bactrocera dorsalis propoxur
3.1.1.7 0.00002777
-
 enzyme from adult, at pH 7.4 and 37°C Bactrocera dorsalis paraoxon-ethyl
3.1.1.7 0.0000428
-
 enzyme from pupa, at pH 7.4 and 37°C Bactrocera dorsalis BW284c51
3.1.1.7 0.0000481
-
 enzyme from pupa, at pH 7.4 and 37°C Bactrocera dorsalis propoxur
3.1.1.7 0.0000658
-
 enzyme from adult, at pH 7.4 and 37°C Bactrocera dorsalis propoxur
3.1.1.7 0.00007052
-
 enzyme from adult, at pH 7.4 and 37°C Bactrocera dorsalis BW284c51
3.1.1.7 0.000086
-
 enzyme from larva, at pH 7.4 and 37°C Bactrocera dorsalis Aldicarb
3.1.1.7 0.000292
-
 enzyme from pupa, at pH 7.4 and 37°C Bactrocera dorsalis Aldicarb
3.1.1.7 0.0004217
-
 enzyme from adult, at pH 7.4 and 37°C Bactrocera dorsalis Aldicarb

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.7 0.8
-
 acetylthiocholine enzyme from adult, at pH 7.4 and 37°C Bactrocera dorsalis
3.1.1.7 2.8
-
 acetylthiocholine enzyme from pupa, at pH 7.4 and 37°C Bactrocera dorsalis
3.1.1.7 20.8
-
 acetylthiocholine enzyme from larva, at pH 7.4 and 37°C Bactrocera dorsalis