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Literature summary extracted from

  • Pingoud, A.; Wilson, G.G.; Wende, W.
    Type II restriction endonucleases-a historical perspective and more (2014), Nucleic Acids Res., 42, 7489-7527.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.4 Mg2+ required Haemophilus influenzae
3.1.21.4 Mg2+ required Escherichia coli
3.1.21.4 Mn2+ Mg2+ can be replaced by Mn2+ albeit with lower efficiency Haemophilus influenzae
3.1.21.4 Mn2+ Mg2+ can be replaced by Mn2+ albeit with lower efficiency Escherichia coli
3.1.21.4 additional information not activated by Ca2+ and Na+ Haemophilus influenzae
3.1.21.4 additional information not activated by Ca2+ and Na+ Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.21.4 30000
-
 x * 30000, EcoRI, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.4 double-stranded DNA + H2O Escherichia coli cleavage by EcoRI is staggered, producing fragments with 4-nucleotide single-stranded overhangs, recognition sequence is GAATTC double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 double-stranded DNA + H2O Escherichia coli cleavage by EcoRV is staggered, producing fragments with 4-nucleotide single-stranded overhangs, recognition sequence is GATATC double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 double-stranded DNA + H2O Haemophilus influenzae cleavage by HindII is blunt, producing fragments with flush ends double-stranded DNA fragments with terminal 5'-phosphates
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.4 Escherichia coli
-
-
-
3.1.21.4 Haemophilus influenzae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.4 double-stranded DNA + H2O cleavage by EcoRI is staggered, producing fragments with 4-nucleotide single-stranded overhangs, recognition sequence is GAATTC Escherichia coli double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 double-stranded DNA + H2O cleavage by EcoRV is staggered, producing fragments with 4-nucleotide single-stranded overhangs, recognition sequence is GATATC Escherichia coli double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 double-stranded DNA + H2O cleavage by HindII is blunt, producing fragments with flush ends Haemophilus influenzae double-stranded DNA fragments with terminal 5'-phosphates
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.21.4 homodimer x * 30000, EcoRI, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.1.21.4 EcoRI
-
 Escherichia coli
3.1.21.4 EcoRV
-
 Escherichia coli
3.1.21.4 HindII
-
 Haemophilus influenzae
3.1.21.4 type II REase
-
 Haemophilus influenzae
3.1.21.4 type II REase
-
 Escherichia coli
3.1.21.4 type II restriction endonuclease
-
 Haemophilus influenzae
3.1.21.4 type II restriction endonuclease
-
 Escherichia coli