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Literature summary extracted from

  • Gorecka, K.M.; Komorowska, W.; Nowotny, M.
    Crystal structure of RuvC resolvase in complex with Holliday junction substrate (2013), Nucleic Acids Res., 41, 9945-9955.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.21.10 expressed in Escherichia coli Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.21.10 in complex with a synthetic Holliday junction, using 0.1 M citric acid (pH 3.5) and 1.5 M NaCl in 0.3-0.5M ammonium phosphate Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.21.10 E70Q inactive Thermus thermophilus
3.1.21.10 H143D the mutant shows increased activity compared to the wild type enzyme Thermus thermophilus
3.1.21.10 L80C inactive Thermus thermophilus
3.1.21.10 M108C the mutant shows reduced activity compared to the wild type enzyme Thermus thermophilus
3.1.21.10 P40C the mutant shows reduced activity compared to the wild type enzyme Thermus thermophilus
3.1.21.10 Q77C inactive Thermus thermophilus
3.1.21.10 R140C the mutant shows reduced activity compared to the wild type enzyme Thermus thermophilus
3.1.21.10 R76C inactive Thermus thermophilus
3.1.21.10 T11C the mutant shows reduced activity compared to the wild type enzyme Thermus thermophilus
3.1.21.10 Y75A the mutant shows markedly increased activity compared to the wild type Thermus thermophilus
3.1.21.10 Y75A/H143D the mutant shows increased activity compared to the wild type enzyme Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.10 additional information requires divalent metal ions for activity Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.10 DNA + H2O Thermus thermophilus
-
hydrolyzed DNA
-
?
3.1.21.10 DNA + H2O Thermus thermophilus DSM 579
-
hydrolyzed DNA
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.10 Thermus thermophilus Q5SJC4
-
-
3.1.21.10 Thermus thermophilus DSM 579 Q5SJC4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.10 nickel affinity column chromatography and Mono-S column chromatography Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.10 DNA + H2O
-
Thermus thermophilus hydrolyzed DNA
-
?
3.1.21.10 DNA + H2O
-
Thermus thermophilus DSM 579 hydrolyzed DNA
-
?

Subunits

EC Number Subunits Comment Organism
3.1.21.10 dimer X-ray crastallography Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
3.1.21.10 Holliday junction resolvase
-
Thermus thermophilus
3.1.21.10 RuvC
-
Thermus thermophilus