EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.21.10 | expressed in Escherichia coli | Thermus thermophilus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.1.21.10 | in complex with a synthetic Holliday junction, using 0.1 M citric acid (pH 3.5) and 1.5 M NaCl in 0.3-0.5M ammonium phosphate | Thermus thermophilus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.21.10 | E70Q | inactive | Thermus thermophilus |
3.1.21.10 | H143D | the mutant shows increased activity compared to the wild type enzyme | Thermus thermophilus |
3.1.21.10 | L80C | inactive | Thermus thermophilus |
3.1.21.10 | M108C | the mutant shows reduced activity compared to the wild type enzyme | Thermus thermophilus |
3.1.21.10 | P40C | the mutant shows reduced activity compared to the wild type enzyme | Thermus thermophilus |
3.1.21.10 | Q77C | inactive | Thermus thermophilus |
3.1.21.10 | R140C | the mutant shows reduced activity compared to the wild type enzyme | Thermus thermophilus |
3.1.21.10 | R76C | inactive | Thermus thermophilus |
3.1.21.10 | T11C | the mutant shows reduced activity compared to the wild type enzyme | Thermus thermophilus |
3.1.21.10 | Y75A | the mutant shows markedly increased activity compared to the wild type | Thermus thermophilus |
3.1.21.10 | Y75A/H143D | the mutant shows increased activity compared to the wild type enzyme | Thermus thermophilus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.21.10 | additional information | requires divalent metal ions for activity | Thermus thermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.21.10 | DNA + H2O | Thermus thermophilus | - |
hydrolyzed DNA | - |
? | |
3.1.21.10 | DNA + H2O | Thermus thermophilus DSM 579 | - |
hydrolyzed DNA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.21.10 | Thermus thermophilus | Q5SJC4 | - |
- |
3.1.21.10 | Thermus thermophilus DSM 579 | Q5SJC4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.21.10 | nickel affinity column chromatography and Mono-S column chromatography | Thermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.21.10 | DNA + H2O | - |
Thermus thermophilus | hydrolyzed DNA | - |
? | |
3.1.21.10 | DNA + H2O | - |
Thermus thermophilus DSM 579 | hydrolyzed DNA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.21.10 | dimer | X-ray crastallography | Thermus thermophilus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.21.10 | Holliday junction resolvase | - |
Thermus thermophilus |
3.1.21.10 | RuvC | - |
Thermus thermophilus |