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Literature summary extracted from
- Structural asymmetry in the Thermus thermophilus RuvC dimer suggests a basis for sequential strand cleavages during Holliday junction resolution (2013), Nucleic Acids Res., 41, 648-656.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.21.10 | expressed in Escherichia coli BL21(DE3) cells | Thermus thermophilus |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.1.21.10 | hanging drop vapor diffusion method, using 35% (w/v) polyethylene glycol 3350, 0.2M Li2SO4 and 0.1M Tris-HCl (pH 8.5) | Thermus thermophilus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.21.10 | D146N | inactive | Thermus thermophilus |
| 3.1.21.10 | F73A | the mutant shows 50% reduced activity compared to the wild type enzyme | Thermus thermophilus |
| 3.1.21.10 | F74A | the mutant shows 30% reduced activity compared to the wild type enzyme | Thermus thermophilus |
| 3.1.21.10 | Y75A | the mutant shows about 1.9fold increase of activity compared to the wild type enzyme | Thermus thermophilus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.21.10 | DNA + H2O | Thermus thermophilus | - |
hydrolyzed DNA | - |
? |  |
| 3.1.21.10 | DNA + H2O | Thermus thermophilus DSM 579 | - |
hydrolyzed DNA | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.21.10 | Thermus thermophilus | Q5SJC4 | - |
- |
| 3.1.21.10 | Thermus thermophilus DSM 579 | Q5SJC4 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.21.10 | Hi-trap heparin-Sepharose column chromatography | Thermus thermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.21.10 | DNA + H2O | - |
Thermus thermophilus | hydrolyzed DNA | - |
? | |
| 3.1.21.10 | DNA + H2O | - |
Thermus thermophilus DSM 579 | hydrolyzed DNA | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.21.10 | homodimer | x-ray crystallography | Thermus thermophilus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.21.10 | HJ resolvase | - |
Thermus thermophilus |
| 3.1.21.10 | Holliday junction resolvase | - |
Thermus thermophilus |
| 3.1.21.10 | RuvC | - |
Thermus thermophilus |
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Release 2023.1 (January 2023)
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