Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Das, U.; Shuman, S.
    Mechanism of RNA 2,3-cyclic phosphate end healing by T4 polynucleotide kinase-phosphatase (2013), Nucleic Acids Res., 41, 355-365.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.78 expressed in Escherichia coli BL21(DE3) cells Tequatrovirus T4
3.1.3.32 expressed in Escherichia coli BL21(DE3) cells Tequatrovirus T4

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.32 D167N the mutant converts a RNA 2',3'-cyclic phosphate end to RNA 3'-phosphate Tequatrovirus T4
3.1.3.32 S211A the mutant transiently accumulates phosphor-RNA during 2',3'-cyclic phosphate removal Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA Tequatrovirus T4
-
 ADP + 5'-phospho-DNA
-
 ?
3.1.3.32 3'-phospho-5'-hydroxy-RNA + H2O Tequatrovirus T4
-
 ?
-
 ?
3.1.3.32 a RNA 2',3'-cyclic phosphate end + H2O Tequatrovirus T4 the enzyme also has RNA 2'-phosphatase activity a RNA 3'-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.78 Tequatrovirus T4
-
-
-
3.1.3.32 Tequatrovirus T4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.78 Ni-agarose column chromatography, and gel filtration Tequatrovirus T4
3.1.3.32 Ni-agarose column chromatography Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA
-
 Tequatrovirus T4 ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 additional information the enzyme also has RNA 2'-phosphatase activity that requires Asp165 and Asp167 Tequatrovirus T4 ?
-
 ?
3.1.3.32 3'-phospho-5'-hydroxy-RNA + H2O
-
 Tequatrovirus T4 ?
-
 ?
3.1.3.32 a RNA 2',3'-cyclic phosphate end + H2O the enzyme also has RNA 2'-phosphatase activity Tequatrovirus T4 a RNA 3'-phosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.78 PNKP
-
 Tequatrovirus T4
2.7.1.78 polynucleotide kinase-phosphatase
-
 Tequatrovirus T4
3.1.3.32 PNKP
-
 Tequatrovirus T4
3.1.3.32 polynucleotide kinase-phosphatase
-
 Tequatrovirus T4