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Literature summary extracted from

  • Ukanis, M.; Sapranauskas, R.; Lubys, A.
    Screening for catalytically active Type II restriction endonucleases using segregation-induced methylation deficiency (2012), Nucleic Acids Res., 40, e149.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.21.4 expressed in Escherichia coli ER2566 cells Citrobacter freundii

Protein Variants

EC Number Protein Variants Comment Organism
3.1.21.4 D134N/K190N/E204V inactive Citrobacter freundii
3.1.21.4 E204Q the mutant possesses 0.004% of wild type activity Citrobacter freundii
3.1.21.4 E71A/K190T/E204I inactive Citrobacter freundii
3.1.21.4 E71A/K190T/E204V inactive Citrobacter freundii
3.1.21.4 E71A/S188A/K190T/E204Q inactive Citrobacter freundii
3.1.21.4 E71N/D254G inactive Citrobacter freundii
3.1.21.4 E71Q the mutant possesses 10% of wild type activity Citrobacter freundii
3.1.21.4 S188A the mutant possesses 4% of wild type activity Citrobacter freundii
3.1.21.4 S188C the mutant possesses 0.5% of wild type activity Citrobacter freundii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.21.4 27000
-
 x * 27000, SDS-PAGE Citrobacter freundii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.4 double-stranded DNA + H2O Citrobacter freundii the enzyme recognizes the sequence 5'-Pu*CCGGPy and cleaves it as indicated by the star double-stranded DNA fragments with terminal 5'-phosphates
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.4 Citrobacter freundii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.4 double-stranded DNA + H2O the enzyme recognizes the sequence 5'-Pu*CCGGPy and cleaves it as indicated by the star Citrobacter freundii double-stranded DNA fragments with terminal 5'-phosphates
-
 ?
3.1.21.4 phage lambda DNA + H2O
-
 Citrobacter freundii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.21.4 ? x * 27000, SDS-PAGE Citrobacter freundii

Synonyms

EC Number Synonyms Comment Organism
3.1.21.4 Cfr10I
-
 Citrobacter freundii
3.1.21.4 type II REase
-
 Citrobacter freundii
3.1.21.4 type II restriction endonuclease
-
 Citrobacter freundii