EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.26.5 | gene At2g47290, DNA and amino acid sequence determination and analysis, AtPop1 mRNA is present in multiple splicing isoforms, possibly encoding several distinct proteins, splicing patterns of AtPop1 mRNAs, overview. Recombinant expression in Escherichia coli strain Rosetta (DE3)pLysS | Arabidopsis thaliana |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.26.5 | nucleus | - |
Triticum aestivum | 5634 | - |
3.1.26.5 | nucleus | - |
Arabidopsis thaliana | 5634 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.26.5 | 21500 | - |
x * 21500, AtPop1 protein, two spliced exons encoding a 190 residues long protein, starting from AUG1 from gene At2G47290, SDS-PAGE | Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.26.5 | additional information | Triticum aestivum | the enzyme processes the 5'-end of tRNAs | ? | - |
? | |
3.1.26.5 | additional information | Arabidopsis thaliana | the enzyme processes the 5'-end of tRNAs | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.26.5 | Arabidopsis thaliana | L0N807 | AtPop1p protein with RNase P activity | - |
3.1.26.5 | Triticum aestivum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.26.5 | recombinant enzyme from Escherichia coli strain Rosetta (DE3)pLysS by nickel affinity chromatography | Arabidopsis thaliana |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.26.5 | germinated grain | wheat germ S23 extract | Triticum aestivum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.26.5 | additional information | the enzyme processes the 5'-end of tRNAs | Triticum aestivum | ? | - |
? | |
3.1.26.5 | additional information | the enzyme processes the 5'-end of tRNAs | Arabidopsis thaliana | ? | - |
? | |
3.1.26.5 | additional information | substrate used in assays is Escherichia coli pre-tRNATyr | Triticum aestivum | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.26.5 | ? | x * 21500, AtPop1 protein, two spliced exons encoding a 190 residues long protein, starting from AUG1 from gene At2G47290, SDS-PAGE | Arabidopsis thaliana |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.26.5 | AtPop1p | - |
Arabidopsis thaliana |
3.1.26.5 | Pop1p | - |
Triticum aestivum |
3.1.26.5 | Pop1p | - |
Arabidopsis thaliana |
3.1.26.5 | RNase P | - |
Triticum aestivum |
3.1.26.5 | RNase P | - |
Arabidopsis thaliana |
3.1.26.5 | RNase P/MRP protein | - |
Triticum aestivum |
3.1.26.5 | RNase P/MRP protein | - |
Arabidopsis thaliana |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.26.5 | 37 | - |
assay at | Triticum aestivum |
3.1.26.5 | 37 | - |
assay at | Arabidopsis thaliana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.26.5 | 7.5 | - |
assay at | Triticum aestivum |
3.1.26.5 | 7.5 | - |
assay at | Arabidopsis thaliana |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.26.5 | additional information | in plants, the protein Pop1p is associated with MRP RNAs, i.e. mitochondrial RNA processing RNAs which cleave the large rRNA precursor at the A3 site, and with the catalytic subunit of enzyme RNase P, either separately or in a single large complex. Pop1p-specific antibodies precipitate RNase P activity from wheat extracts. The eukaryotic RNase P consensus sequence with CR II and CR III that are signature elements specific for RNase P RNA | Triticum aestivum |
3.1.26.5 | additional information | in plants, the protein Pop1p is associated with MRP RNAs, i.e. mitochondrial RNA processing RNAs which cleave the large rRNA precursor at the A3 site, and with the catalytic subunit of enzyme RNase P, either separately or in a single large complex. The eukaryotic RNase P consensus sequence with CR II and CR III that are signature elements specific for RNase P RNA | Arabidopsis thaliana |