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Literature summary extracted from
- Structural basis for cofactor-independent dioxygenation in vancomycin biosynthesis (2007), Nature, 447, 342-345.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.13.11.80 | - |
Streptomyces toyocaensis |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.13.11.80 | structure of the enzyme in complex with a bound substrate mimic, crystallized by the hanging drop method at 20°C, 2.75 A resolution | Streptomyces toyocaensis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.13.11.80 | L237T | kcat/KM for (3,5-dihydroxyphenyl)acetyl-CoA is 2% compared to the wild-type activity | Streptomyces toyocaensis |
| 1.13.11.80 | V425T | kcat/KM for (3,5-dihydroxyphenyl)acetyl-CoA is 6% compared to the wild-type activity | Streptomyces toyocaensis |
| 1.13.11.80 | V429T | kcat/KM for (3,5-dihydroxyphenyl)acetyl-CoA is 3% compared to the wild-type activity | Streptomyces toyocaensis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.80 | [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]methyl (3R)-4-([3-[(2-[[(2,5-dihydroxyphenyl)acetyl]amino]ethyl)amino]-3-oxopropyl]amino)-3-hydroxy-2,2-dimethyl-4-oxobutyl dihydrogen diphosphate | - |
Streptomyces toyocaensis |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.80 | 0.0017 | - |
O2 | pH 7.4, 24°C | Streptomyces toyocaensis | |
| 1.13.11.80 | 0.0021 | - |
O2 | pH 7.4, 24°C | Streptomyces toyocaensis | |
| 1.13.11.80 | 0.0026 | - |
O2 | pH 7.4, 24°C | Streptomyces toyocaensis | |
| 1.13.11.80 | 0.0039 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | pH 7.4, 24°C | Streptomyces toyocaensis | |
| 1.13.11.80 | 0.0138 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | pH 7.4, 24°C | Streptomyces toyocaensis | |
| 1.13.11.80 | 0.0147 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | pH 7.4, 24°C | Streptomyces toyocaensis | |
| 1.13.11.80 | 0.058 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | pH 7.4, 24°C | Streptomyces toyocaensis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.80 | additional information | not dependent on a metal ion | Streptomyces toyocaensis |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.80 | 354000 | - |
gel filtration | Streptomyces toyocaensis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.80 | (3,5-dihydroxyphenyl)acetyl-CoA + O2 | Streptomyces toyocaensis | the enzyme is involved in the biosynthesis of vancomycin and teicoplanin antibiotics. (3,5-dihydroxyphenyl)acetyl-CoA i.e. 2-(3,5-dihydroxyphenyl)acetyl-CoA | (3,5-dihydroxyphenyl)glyoxylate + CoA | - |
? | |
| 1.13.11.80 | (3,5-dihydroxyphenyl)acetyl-CoA + O2 | Streptomyces toyocaensis A47934 | the enzyme is involved in the biosynthesis of vancomycin and teicoplanin antibiotics. (3,5-dihydroxyphenyl)acetyl-CoA i.e. 2-(3,5-dihydroxyphenyl)acetyl-CoA | (3,5-dihydroxyphenyl)glyoxylate + CoA | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.80 | Streptomyces toyocaensis | Q8KLK7 | - |
- |
| 1.13.11.80 | Streptomyces toyocaensis A47934 | Q8KLK7 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.11.80 | - |
Streptomyces toyocaensis |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.13.11.80 | (3,5-dihydroxyphenyl)acetyl-CoA + O2 = 2-(3,5-dihydroxyphenyl)-2-oxoacetate + CoA | proposed catalytic mechanism | Streptomyces toyocaensis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.80 | (3,5-dihydroxyphenyl)acetyl-CoA + O2 | the enzyme is involved in the biosynthesis of vancomycin and teicoplanin antibiotics. (3,5-dihydroxyphenyl)acetyl-CoA i.e. 2-(3,5-dihydroxyphenyl)acetyl-CoA | Streptomyces toyocaensis | (3,5-dihydroxyphenyl)glyoxylate + CoA | - |
? | |
| 1.13.11.80 | (3,5-dihydroxyphenyl)acetyl-CoA + O2 | (3,5-dihydroxyphenyl)acetyl-CoA i.e. 2-(3,5-dihydroxyphenyl)acetyl-CoA | Streptomyces toyocaensis | (3,5-dihydroxyphenyl)glyoxylate + CoA | - |
? | |
| 1.13.11.80 | (3,5-dihydroxyphenyl)acetyl-CoA + O2 | the enzyme is involved in the biosynthesis of vancomycin and teicoplanin antibiotics. (3,5-dihydroxyphenyl)acetyl-CoA i.e. 2-(3,5-dihydroxyphenyl)acetyl-CoA | Streptomyces toyocaensis A47934 | (3,5-dihydroxyphenyl)glyoxylate + CoA | - |
? | |
| 1.13.11.80 | (3,5-dihydroxyphenyl)acetyl-CoA + O2 | (3,5-dihydroxyphenyl)acetyl-CoA i.e. 2-(3,5-dihydroxyphenyl)acetyl-CoA | Streptomyces toyocaensis A47934 | (3,5-dihydroxyphenyl)glyoxylate + CoA | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.13.11.80 | homohexamer | crystallographic data | Streptomyces toyocaensis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.13.11.80 | DpgC | - |
Streptomyces toyocaensis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.80 | 24 | - |
assay at | Streptomyces toyocaensis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.80 | 0.019 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | pH 7.4, 24°C | Streptomyces toyocaensis | |
| 1.13.11.80 | 0.037 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | pH 7.4, 24°C | Streptomyces toyocaensis | |
| 1.13.11.80 | 0.05 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | pH 7.4, 24°C | Streptomyces toyocaensis | |
| 1.13.11.80 | 0.172 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | pH 7.4, 24°C | Streptomyces toyocaensis | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.80 | 7.5 | - |
assay at | Streptomyces toyocaensis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.80 | additional information | no cofactor detected | Streptomyces toyocaensis |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.80 | 0.0027 | - |
[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]methyl (3R)-4-([3-[(2-[[(2,5-dihydroxyphenyl)acetyl]amino]ethyl)amino]-3-oxopropyl]amino)-3-hydroxy-2,2-dimethyl-4-oxobutyl dihydrogen diphosphate | pH 7.4, 24°C | Streptomyces toyocaensis | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.13.11.80 | physiological function | the enzyme is involved in the biosynthesis of vancomycin and teicoplanin antibiotics | Streptomyces toyocaensis |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.80 | 0.86 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | pH 7.4, 24°C | Streptomyces toyocaensis | |
| 1.13.11.80 | 1.3 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | pH 7.4, 24°C | Streptomyces toyocaensis | |
| 1.13.11.80 | 2.7 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | pH 7.4, 24°C | Streptomyces toyocaensis | |
| 1.13.11.80 | 44 | - |
(3,5-dihydroxyphenyl)acetyl-CoA | pH 7.4, 24°C | Streptomyces toyocaensis | |
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