EC Number | Cloned (Comment) | Organism |
---|---|---|
4.3.1.2 | expressed in Escherichia coli | Clostridium tetanomorphum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.3.1.2 | L384A | mutant displays pronounced amination activity towards 2-hexylfumarate ad decreased amination activity towards mesaconate compared to wild-type. Mutant displays a broad electrophile scope including fumarate derivatives with alkyl, aryl, alkoxy, aryloxy, alkylthio and arylthio substituents at the C2 position | Clostridium tetanomorphum |
4.3.1.2 | Q73A | mutant displays significant decrease in ammonia addition and a large increase in methylamine addition compared to wild-type. Mutant displays a wide nucleophile scope including structurally diverse linear and cyclic allkylamines | Clostridium tetanomorphum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.3.1.2 | Mg2+ | - |
Clostridium tetanomorphum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.3.1.2 | Clostridium tetanomorphum | Q05514 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.1.2 | (2E)-2-butylbut-2-enedioate + NH3 | wild-type: conversion 0%, mutant L384A: conversion 59% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | (2E)-2-ethylbut-2-enedioate + NH3 | wild-type: conversion 74%, mutant L384A: conversion 76% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | (2E)-2-hexylbut-2-enedioate + NH3 | wild-type: conversion 0%, mutant L384A: conversion 53% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | (2E)-2-methylbut-2-enedioate + NH3 | wild-type: conversion 80%, mutant L384A: conversion 75% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | (2E)-2-pentylbut-2-enedioate + NH3 | wild-type: conversion 0%, mutant L384A: conversion 52% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | (2E)-2-phenylbut-2-enedioate + NH3 | wild-type: conversion 0%, mutant L384A: conversion 90% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | (2E)-2-propylbut-2-enedioate + NH3 | wild-type: conversion 57%, mutant L384A: conversion 67% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | (2Z)-2-(benzyloxy)but-2-enedioate + NH3 | wild-type: conversion 0%, mutant L384A: conversion 60% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | (2Z)-2-(benzylsulfanyl)but-2-enedioate + NH3 | wild-type: conversion 0%, mutant L384A: conversion 42% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | (2Z)-2-(ethylsulfanyl)but-2-enedioate + NH3 | wild-type: conversion 36%, mutant L384A: conversion 50% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | (2Z)-2-(phenylsulfanyl)but-2-enedioate + NH3 | wild-type: conversion 0%, mutant L384A: conversion 34% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | (2Z)-2-ethoxybut-2-enedioate + NH3 | wild-type: conversion 46%, mutant L384A: conversion 43% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | (2Z)-2-phenoxybut-2-enedioate + NH3 | wild-type: conversion 0%, mutant L384A: conversion 44% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | mesaconate + (aminooxy)methane | mutant Q73A, conversion: 99%, excess of threo isomer over erythro isomer: more than 95% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | mesaconate + 1-cyclopropylmethanamine | mutant Q73A, conversion: 61%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | mesaconate + 2-aminoethanol | mutant Q73A, conversion: 37%, excess of threo isomer over erythro isomer: more than 95% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | mesaconate + 2-methoxyethanamine | mutant Q73A, conversion: 60%, excess of threo isomer over erythro isomer: more than 95% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | mesaconate + 3-aminopropan-1-ol | mutant Q73A, conversion: 56%, excess of threo isomer over erythro isomer: 80% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | mesaconate + aminobutane | mutant Q73A, conversion: 60%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | mesaconate + aniline | mutant Q73A, conversion: 6%, excess of threo isomer over erythro isomer: more than 95% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | mesaconate + cyclobutanamine | mutant Q73A, conversion: 65%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | mesaconate + cyclohexanamine | mutant Q73A, conversion: 21%, excess of threo isomer over erythro isomer: more than 95% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | mesaconate + cyclopentanamine | mutant Q73A, conversion: 36%, excess of threo isomer over erythro isomer: more than 95% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | mesaconate + cyclopropanamine | mutant Q73A, conversion: 75%, excess of threo isomer over erythro isomer: more than 95% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | mesaconate + ethanamine | mutant Q73A, conversion: 51%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | mesaconate + ethane-1,2-diamine | mutant Q73A, conversion: 90%, excess of threo isomer over erythro isomer: more than 95% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | mesaconate + hexanamine | mutant Q73A, conversion: 17%, excess of threo isomer over erythro isomer: more than 95% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | mesaconate + methanamine | mutant Q73A, conversion: 74%, excess of threo isomer over erythro isomer: more than 95% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | mesaconate + N-methylethane-1,2-diamine | mutant Q73A, conversion: 80%, excess of threo isomer over erythro isomer: more than 95% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | mesaconate + NH3 | mutant Q73A, conversion: 79%, excess of threo isomer over erythro isomer: 5% | Clostridium tetanomorphum | L-threo-3-methylaspartate | - |
r | |
4.3.1.2 | mesaconate + NH3 | wild-type: conversion 100%, mutant L384A: conversion 99% | Clostridium tetanomorphum | L-threo-3-methylaspartate | - |
r | |
4.3.1.2 | mesaconate + pentanamine | mutant Q73A, conversion: 29%, excess of threo isomer over erythro isomer: more than 95% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | mesaconate + propan-2-amine | mutant Q73A, conversion: 8%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | mesaconate + propanamine | mutant Q73A, conversion: 53%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99% | Clostridium tetanomorphum | ? | - |
r | |
4.3.1.2 | mesaconate + propane-1,3-diamine | mutant Q73A, conversion: 78%, excess of threo isomer over erythro isomer: more than 95% | Clostridium tetanomorphum | ? | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.3.1.2 | MAL | - |
Clostridium tetanomorphum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.3.1.2 | 22 | - |
assay at | Clostridium tetanomorphum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.3.1.2 | 9 | - |
assay at | Clostridium tetanomorphum |