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Literature summary extracted from

  • Bali, S.; Rollauer, S.; Roversi, P.; Raux-Deery, E.; Lea, S.M.; Warren, M.J.; Ferguson, S.J.
    Identification and characterization of the missing terminal enzyme for siroheme biosynthesis in alpha-proteobacteria (2014), Mol. Microbiol., 92, 153-163.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.99.1.3 expressed in Escherichia coli BL21 codonplus (RIPL) cells Paracoccus pantotrophus
4.99.1.4 expressed in Escherichia coli BL21 codonplus (RIPL) cells Paracoccus pantotrophus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.99.1.4 sitting drop vapor diffusion method, using in 0.2 M imidazole malate pH 5.5, 24% (w/v) PEG 600 at a ratio of 50:50 ratio of protein : mother liquor and 10% ethylene glycol Paracoccus pantotrophus

Protein Variants

EC Number Protein Variants Comment Organism
4.99.1.3 H127A inactive Paracoccus pantotrophus
4.99.1.3 H187A inactive Paracoccus pantotrophus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.99.1.3 23000
-
 x * 23000, SDS-PAGE Paracoccus pantotrophus
4.99.1.4 20000
-
 x * 20000, SDS-PAGE Paracoccus pantotrophus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.99.1.3 cobalt-sirohydrochlorin + H+ Paracoccus denitrificans
-
 sirohydrochlorin + Co2+
-
 ?
4.99.1.3 cobalt-sirohydrochlorin + H+ Paracoccus pantotrophus
-
 sirohydrochlorin + Co2+
-
 ?
4.99.1.4 sirohydrochlorin + Fe2+ Paracoccus pantotrophus
-
 siroheme + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.99.1.3 Paracoccus denitrificans
-
-
-
4.99.1.3 Paracoccus pantotrophus
-
-
-
4.99.1.4 Paracoccus pantotrophus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.99.1.3 Ni-Sepharose column chromatography Paracoccus pantotrophus
4.99.1.4 chelating Sepharose column chromatography Paracoccus pantotrophus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.99.1.3 cobalt-sirohydrochlorin + H+
-
 Paracoccus denitrificans sirohydrochlorin + Co2+
-
 ?
4.99.1.3 cobalt-sirohydrochlorin + H+
-
 Paracoccus pantotrophus sirohydrochlorin + Co2+
-
 ?
4.99.1.4 sirohydrochlorin + Fe2+
-
 Paracoccus pantotrophus siroheme + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
4.99.1.3 ? x * 23000, SDS-PAGE Paracoccus pantotrophus
4.99.1.4 ? x * 20000, SDS-PAGE Paracoccus pantotrophus

Synonyms

EC Number Synonyms Comment Organism
4.99.1.3 CbiX
-
 Paracoccus denitrificans
4.99.1.3 CbiX
-
 Paracoccus pantotrophus
4.99.1.3 cobaltochelatase
-
 Paracoccus denitrificans
4.99.1.3 cobaltochelatase
-
 Paracoccus pantotrophus
4.99.1.3 sirohydrochlorin-ferrochelatase
-
 Paracoccus denitrificans
4.99.1.3 sirohydrochlorin-ferrochelatase
-
 Paracoccus pantotrophus
4.99.1.4 CbiX
-
 Paracoccus pantotrophus
4.99.1.4 CysG
-
 Paracoccus pantotrophus
4.99.1.4 Met8p
-
 Paracoccus pantotrophus
4.99.1.4 SirB
-
 Paracoccus pantotrophus
4.99.1.4 sirohydrochlorin-ferrochelatase
-
 Paracoccus pantotrophus

General Information

EC Number General Information Comment Organism
4.99.1.3 malfunction a cbiX-deficient mutant is unable to respire anaerobically on nitrate Paracoccus denitrificans