EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.8.12 | expression in Escherichia coli as a N-terminal histidine-tagged fusion protein | Cereibacter sphaeroides |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.8.12 | 62300 | - |
2 * 62300, N-terminal histidine-tagged fusion protein, SDS-PAGE | Cereibacter sphaeroides |
1.3.8.12 | 100000 | - |
gel filtration | Cereibacter sphaeroides |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.8.12 | (2S)-methylsuccinyl-CoA + electron-transfer flavoprotein | Cereibacter sphaeroides | the enzyme is involved ion the ethylmalonyl-CoA pathway for acetyl-CoA assimilation | 2-methylfumaryl-CoA + reduced electron-transfer flavoprotein | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.8.12 | Cereibacter sphaeroides | D3JV03 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.8.12 | - |
Cereibacter sphaeroides |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.3.8.12 | culture condition:acetate-grown cell | - |
Cereibacter sphaeroides | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.8.12 | (2S)-methylsuccinyl-CoA + electron-transfer flavoprotein | the enzyme is involved ion the ethylmalonyl-CoA pathway for acetyl-CoA assimilation | Cereibacter sphaeroides | 2-methylfumaryl-CoA + reduced electron-transfer flavoprotein | - |
? | |
1.3.8.12 | (2S)-methylsuccinyl-CoA + electron-transfer flavoprotein | the enzyme is highly specific for (S)-methylsuccinyl-CoA. No activity with butyryl-CoA, isobutyryl-CoA or a diastereomeric mixture of (R)-methylsuccinyl-CoA | Cereibacter sphaeroides | 2-methylfumaryl-CoA + reduced electron-transfer flavoprotein | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.8.12 | homodimer | 2 * 62300, N-terminal histidine-tagged fusion protein, SDS-PAGE | Cereibacter sphaeroides |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.8.12 | MCD | - |
Cereibacter sphaeroides |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.8.12 | 30 | - |
assay at | Cereibacter sphaeroides |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.8.12 | 7.8 | - |
assay at | Cereibacter sphaeroides |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.8.12 | FAD | the enzyme contains 0.7 mol of noncovalently bound FAD molecule per subunit | Cereibacter sphaeroides |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.3.8.12 | metabolism | the enzyme is involved in the ethylmalonyl-CoA pathway, an acetyl-CoA assimilation strategy | Cereibacter sphaeroides |