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Literature summary extracted from

  • Erb, T.J.; Fuchs, G.; Alber, B.E.
    (2S)-Methylsuccinyl-CoA dehydrogenase closes the ethylmalonyl-CoA pathway for acetyl-CoA assimilation (2009), Mol. Microbiol., 73, 992-1008.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.8.12 expression in Escherichia coli as a N-terminal histidine-tagged fusion protein Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.8.12 62300
-
2 * 62300, N-terminal histidine-tagged fusion protein, SDS-PAGE Cereibacter sphaeroides
1.3.8.12 100000
-
gel filtration Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.8.12 (2S)-methylsuccinyl-CoA + electron-transfer flavoprotein Cereibacter sphaeroides the enzyme is involved ion the ethylmalonyl-CoA pathway for acetyl-CoA assimilation 2-methylfumaryl-CoA + reduced electron-transfer flavoprotein
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.12 Cereibacter sphaeroides D3JV03
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.8.12
-
Cereibacter sphaeroides

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.8.12 culture condition:acetate-grown cell
-
Cereibacter sphaeroides
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.12 (2S)-methylsuccinyl-CoA + electron-transfer flavoprotein the enzyme is involved ion the ethylmalonyl-CoA pathway for acetyl-CoA assimilation Cereibacter sphaeroides 2-methylfumaryl-CoA + reduced electron-transfer flavoprotein
-
?
1.3.8.12 (2S)-methylsuccinyl-CoA + electron-transfer flavoprotein the enzyme is highly specific for (S)-methylsuccinyl-CoA. No activity with butyryl-CoA, isobutyryl-CoA or a diastereomeric mixture of (R)-methylsuccinyl-CoA Cereibacter sphaeroides 2-methylfumaryl-CoA + reduced electron-transfer flavoprotein
-
?

Subunits

EC Number Subunits Comment Organism
1.3.8.12 homodimer 2 * 62300, N-terminal histidine-tagged fusion protein, SDS-PAGE Cereibacter sphaeroides

Synonyms

EC Number Synonyms Comment Organism
1.3.8.12 MCD
-
Cereibacter sphaeroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.8.12 30
-
assay at Cereibacter sphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.8.12 7.8
-
assay at Cereibacter sphaeroides

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.8.12 FAD the enzyme contains 0.7 mol of noncovalently bound FAD molecule per subunit Cereibacter sphaeroides

General Information

EC Number General Information Comment Organism
1.3.8.12 metabolism the enzyme is involved in the ethylmalonyl-CoA pathway, an acetyl-CoA assimilation strategy Cereibacter sphaeroides