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Literature summary extracted from

  • Burns, K.E.; Cerda-Maira, F.A.; Wang, T.; Li, H.; Bishai, W.R.; Darwin, K.H.
    "Depupylation" of prokaryotic ubiquitin-like protein from mycobacterial proteasome substrates (2010), Mol. Cell., 39, 821-827.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.119 recombinant His6-tagged enzyme is highly insoluble when overproduced in Escherichia coli Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.119 [prokaryotic ubiquitin-like protein]-L-glutamine + H2O Mycobacterium tuberculosis the enzyme deamidates the carboxyl-terminal glutamine of Pup to glutamate, preparing it for ligation to target proteins by proteasome accessory factor A (PafA) [prokaryotic ubiquitin-like protein]-L-glutamate + NH3
-
 ?
3.5.1.119 [prokaryotic ubiquitin-like protein]-L-glutamine + H2O Mycobacterium tuberculosis ATCC 25618 the enzyme deamidates the carboxyl-terminal glutamine of Pup to glutamate, preparing it for ligation to target proteins by proteasome accessory factor A (PafA) [prokaryotic ubiquitin-like protein]-L-glutamate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.119 Mycobacterium tuberculosis P9WNU9
-
-
3.5.1.119 Mycobacterium tuberculosis ATCC 25618 P9WNU9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.119 additional information the enzyme is unable to hydrolyze linear Pup-substrate fusions Mycobacterium tuberculosis ?
-
 ?
3.5.1.119 additional information the enzyme is unable to hydrolyze linear Pup-substrate fusions Mycobacterium tuberculosis ATCC 25618 ?
-
 ?
3.5.1.119 N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[His6-myo-inositol-1-phosphate synthetase]-L-lysine + H2O
-
 Mycobacterium tuberculosis [prokaryotic ubiquitin-like protein]-L-glutamate + [His6-myo-inositol-1-phosphate synthetase]-L-lysine
-
 ?
3.5.1.119 N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[His6-myo-inositol-1-phosphate synthetase]-L-lysine + H2O
-
 Mycobacterium tuberculosis ATCC 25618 [prokaryotic ubiquitin-like protein]-L-glutamate + [His6-myo-inositol-1-phosphate synthetase]-L-lysine
-
 ?
3.5.1.119 N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[malonyl Co-A acyl carrier protein transacylase]-L-lysine + H2O
-
 Mycobacterium tuberculosis [prokaryotic ubiquitin-like protein]-L-glutamate + [malonyl Co-A acyl carrier protein transacylase]-L-lysine
-
 ?
3.5.1.119 N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[malonyl Co-A acyl carrier protein transacylase]-L-lysine + H2O
-
 Mycobacterium tuberculosis ATCC 25618 [prokaryotic ubiquitin-like protein]-L-glutamate + [malonyl Co-A acyl carrier protein transacylase]-L-lysine
-
 ?
3.5.1.119 [prokaryotic ubiquitin-like protein]-L-glutamine + H2O the enzyme deamidates the carboxyl-terminal glutamine of Pup to glutamate, preparing it for ligation to target proteins by proteasome accessory factor A (PafA) Mycobacterium tuberculosis [prokaryotic ubiquitin-like protein]-L-glutamate + NH3
-
 ?
3.5.1.119 [prokaryotic ubiquitin-like protein]-L-glutamine + H2O the enzyme deamidates the carboxyl-terminal glutamine of Pup to glutamate Mycobacterium tuberculosis [prokaryotic ubiquitin-like protein]-L-glutamate + NH3
-
 ?
3.5.1.119 [prokaryotic ubiquitin-like protein]-L-glutamine + H2O the enzyme deamidates the carboxyl-terminal glutamine of Pup to glutamate, preparing it for ligation to target proteins by proteasome accessory factor A (PafA) Mycobacterium tuberculosis ATCC 25618 [prokaryotic ubiquitin-like protein]-L-glutamate + NH3
-
 ?
3.5.1.119 [prokaryotic ubiquitin-like protein]-L-glutamine + H2O the enzyme deamidates the carboxyl-terminal glutamine of Pup to glutamate Mycobacterium tuberculosis ATCC 25618 [prokaryotic ubiquitin-like protein]-L-glutamate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.119 deamidase of Pup
-
 Mycobacterium tuberculosis
3.5.1.119 Dop
-
 Mycobacterium tuberculosis
3.5.1.119 DPUP
-
 Mycobacterium tuberculosis
3.5.1.119 Rv2112c locus name Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
3.5.1.119 ATP the reaction is ATP-dependent, but ATP is not hydrolyzed during the course of the reaction. Both adenosine-5'-[(alpha,beta)-methylene]triphosphate and adenosine 5'-(beta,gamma-imido)triphosphate (AMP-PNP), non-hydrolyzable ATP analogues, can substitute for ATP Mycobacterium tuberculosis