Literature summary extracted from

Cayir, E.; Erdemir, A.; Ozkan, E.; Topuzogullari, M.; Bolat, Z.B.; Akat, A.; Turgut-Balik, D.
Cloning of intron-removed enolase gene and expression, purification, kinetic characterization of the enzyme from Theileria annulata (2014), Mol. Biotechnol., 56, 689-696.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
4.2.1.11	expressed in Escherichia coli BL21(DE3) cells	Theileria annulata

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.2.1.11	0.106	-	2-phospho-D-glycerate	1.5 mM MgCl2 in 50 mM Tris/HCl (pH 7.4), at 25°C	Theileria annulata

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
4.2.1.11	48000	-	2 * 48000, SDS-PAGE	Theileria annulata
4.2.1.11	93000	-	gel filtration	Theileria annulata

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4.2.1.11	2-phospho-D-glycerate	Theileria annulata	-	phosphoenolpyruvate + H2O	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.2.1.11	Theileria annulata	E9NJD0	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
4.2.1.11	Ni-NTA agarose column chromatography	Theileria annulata

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.2.1.11	2-phospho-D-glycerate	-	Theileria annulata	phosphoenolpyruvate + H2O	-	?

Subunits

EC Number	Subunits	Comment	Organism
4.2.1.11	homodimer	2 * 48000, SDS-PAGE	Theileria annulata

Synonyms

EC Number	Synonyms	Comment	Organism
4.2.1.11	enolase	-	Theileria annulata

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4.2.1.11	37	-	2-phospho-D-glycerate	1.5 mM MgCl2 in 50 mM Tris/HCl (pH 7.4), at 25°C	Theileria annulata

kcat/KM [mM/s]

EC Number	kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
4.2.1.11	350	-	2-phospho-D-glycerate	1.5 mM MgCl2 in 50 mM Tris/HCl (pH 7.4), at 25°C	Theileria annulata

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Release 2023.1 (January 2023)