BRENDA - Enzyme Database

Cloning of intron-removed enolase gene and expression, purification, kinetic characterization of the enzyme from Theileria annulata

Cayir, E.; Erdemir, A.; Ozkan, E.; Topuzogullari, M.; Bolat, Z.B.; Akat, A.; Turgut-Balik, D.; Mol. Biotechnol. 56, 689-696 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli BL21(DE3) cells
Theileria annulata
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.106
-
2-phospho-D-glycerate
1.5 mM MgCl2 in 50 mM Tris/HCl (pH 7.4), at 25°C
Theileria annulata
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
48000
-
2 * 48000, SDS-PAGE
Theileria annulata
4.2.1.11
93000
-
gel filtration
Theileria annulata
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Theileria annulata
-
phosphoenolpyruvate + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Theileria annulata
E9NJD0
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
Ni-NTA agarose column chromatography
Theileria annulata
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
730374
Theileria annulata
phosphoenolpyruvate + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
homodimer
2 * 48000, SDS-PAGE
Theileria annulata
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.11
37
-
2-phospho-D-glycerate
1.5 mM MgCl2 in 50 mM Tris/HCl (pH 7.4), at 25°C
Theileria annulata
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli BL21(DE3) cells
Theileria annulata
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.106
-
2-phospho-D-glycerate
1.5 mM MgCl2 in 50 mM Tris/HCl (pH 7.4), at 25°C
Theileria annulata
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
48000
-
2 * 48000, SDS-PAGE
Theileria annulata
4.2.1.11
93000
-
gel filtration
Theileria annulata
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Theileria annulata
-
phosphoenolpyruvate + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
Ni-NTA agarose column chromatography
Theileria annulata
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
730374
Theileria annulata
phosphoenolpyruvate + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
homodimer
2 * 48000, SDS-PAGE
Theileria annulata
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.11
37
-
2-phospho-D-glycerate
1.5 mM MgCl2 in 50 mM Tris/HCl (pH 7.4), at 25°C
Theileria annulata
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.2.1.11
350
-
2-phospho-D-glycerate
1.5 mM MgCl2 in 50 mM Tris/HCl (pH 7.4), at 25°C
Theileria annulata
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.2.1.11
350
-
2-phospho-D-glycerate
1.5 mM MgCl2 in 50 mM Tris/HCl (pH 7.4), at 25°C
Theileria annulata