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Literature summary extracted from

  • Su, R.R.; Wang, A.; Hou, S.T.; Gao, P.; Zhu, G.P.; Wang, W.
    Identification of a novel fumarase C from Streptomyces lividans TK54 as a good candidate for L-malate production (2014), Mol. Biol. Rep., 41, 497-504.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.2 phosphate
-
 Streptomyces lividans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.2 expressed in Escherichia coli Streptomyces lividans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.2 6.7
-
 L-malate pH 6.5, 40°C Streptomyces lividans
4.2.1.2 15
-
 L-malate pH 7.3, 40°C Streptomyces lividans
4.2.1.2 57
-
 L-malate pH 8, 40°C Streptomyces lividans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.2 49000
-
 SDS-PAGE Streptomyces lividans

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.2 Streptomyces lividans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.2 fumarate + H2O
-
 Streptomyces lividans L-malate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.2 fumarase C
-
 Streptomyces lividans
4.2.1.2 slFumC
-
 Streptomyces lividans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.2 45
-
-
 Streptomyces lividans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.2 40
-
 over 85% of activity after 48 h incubation at 40°C Streptomyces lividans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.2 65
-
 L-malate pH 6.5, 40°C Streptomyces lividans
4.2.1.2 267
-
 L-malate pH 7.3, 40°C Streptomyces lividans
4.2.1.2 620
-
 L-malate pH 8, 40°C Streptomyces lividans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.2 8
-
-
 Streptomyces lividans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.2 9.7
-
 L-malate pH 6.5, 40°C Streptomyces lividans
4.2.1.2 10.88
-
 L-malate pH 8, 40°C Streptomyces lividans
4.2.1.2 17.8
-
 L-malate pH 7.3, 40°C Streptomyces lividans