BRENDA - Enzyme Database

Importance of enolase in Giardia lamblia differentiation

Castillo-Romero, A.; Davids, B.J.; Lauwaet, T.; Gillin, F.D.; Mol. Biochem. Parasitol. 184, 122-125 (2012)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
D257K
the mutation has no effect on excystation
Giardia intestinalis
4.2.1.11
H389Q/R390S
the mutations significantly inhibit excystation
Giardia intestinalis
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cell membrane
-
Giardia intestinalis
-
-
4.2.1.11
cytosol
-
Giardia intestinalis
5829
-
4.2.1.11
flagellum
caudal flagellum
Giardia intestinalis
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
Mg2+ is the strongest enolase activating metal. Binding of two Mg2+ ions is essential
Giardia intestinalis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
48000
-
x * 48000, SDS-PAGE
Giardia intestinalis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Giardia intestinalis
-
phosphoenolpyruvate + H2O
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Giardia intestinalis
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
730352
Giardia intestinalis
phosphoenolpyruvate + H2O
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 48000, SDS-PAGE
Giardia intestinalis
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
D257K
the mutation has no effect on excystation
Giardia intestinalis
4.2.1.11
H389Q/R390S
the mutations significantly inhibit excystation
Giardia intestinalis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cell membrane
-
Giardia intestinalis
-
-
4.2.1.11
cytosol
-
Giardia intestinalis
5829
-
4.2.1.11
flagellum
caudal flagellum
Giardia intestinalis
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
Mg2+ is the strongest enolase activating metal. Binding of two Mg2+ ions is essential
Giardia intestinalis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
48000
-
x * 48000, SDS-PAGE
Giardia intestinalis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Giardia intestinalis
-
phosphoenolpyruvate + H2O
-
-
r
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
730352
Giardia intestinalis
phosphoenolpyruvate + H2O
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 48000, SDS-PAGE
Giardia intestinalis