Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Baothman, O.A.; Rolfe, M.D.; Green, J.
    Characterization of Salmonella enterica serovar Typhimurium aconitase A (2013), Microbiology, 159, 1209-1216.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.3 expressed in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.3 0.2
-
 cis-aconitate pH 8, 25°C, Lineweaver-Burk method Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.3 0.9
-
 isocitrate pH 8, 25°C, Lineweaver-Burk method Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.3 5.3
-
 citrate pH 8, 25°C, Lineweaver-Burk method Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.3 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.3 using Ni-NTA chromatography Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.3 cis-aconitate + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium isocitrate
-
 ?
4.2.1.3 citrate
-
 Salmonella enterica subsp. enterica serovar Typhimurium cis-aconitate + H2O
-
 ?
4.2.1.3 isocitrate
-
 Salmonella enterica subsp. enterica serovar Typhimurium cis-aconitate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.3 monomer gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

EC Number Synonyms Comment Organism
4.2.1.3 Acn
-
 Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.3 aconitase A
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.3 25
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.3 1.1
-
 isocitrate pH 8, 25°C, Lineweaver-Burk method Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.3 5.2
-
 cis-aconitate pH 8, 25°C, Lineweaver-Burk method Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.3 5.3
-
 citrate pH 8, 25°C, Lineweaver-Burk method Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.3 8
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

Expression

EC Number Organism Comment Expression
4.2.1.3 Salmonella enterica subsp. enterica serovar Typhimurium expression of acnA is repressed by the presence of acnB down
4.2.1.3 Salmonella enterica subsp. enterica serovar Typhimurium promoter fusion experiments show that acnA expression is regulated by the cyclic-AMP receptor protein, the fumarate nitrate reduction regulator, the ferric uptake regulator and the superoxide response protein additional information
4.2.1.3 Salmonella enterica subsp. enterica serovar Typhimurium acnA expression is enhanced in an acnB mutant up

General Information

EC Number General Information Comment Organism
4.2.1.3 malfunction acnA expression is enhanced in an acnB mutant Salmonella enterica subsp. enterica serovar Typhimurium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.3 1
-
 citrate pH 8, 25°C, Lineweaver-Burk method Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.3 1.2
-
 isocitrate pH 8, 25°C, Lineweaver-Burk method Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.3 26
-
 cis-aconitate pH 8, 25°C, Lineweaver-Burk method Salmonella enterica subsp. enterica serovar Typhimurium