BRENDA - Enzyme Database

Characterization of Salmonella enterica serovar Typhimurium aconitase A

Baothman, O.A.; Rolfe, M.D.; Green, J.; Microbiology 159, 1209-1216 (2013)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.3
expressed in Escherichia coli
Salmonella enterica subsp. enterica serovar Typhimurium
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.3
0.2
-
cis-aconitate
pH 8, 25°C, Lineweaver-Burk method
Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.3
0.9
-
isocitrate
pH 8, 25°C, Lineweaver-Burk method
Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.3
5.3
-
citrate
pH 8, 25°C, Lineweaver-Burk method
Salmonella enterica subsp. enterica serovar Typhimurium
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.3
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.3
using Ni-NTA chromatography
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
cis-aconitate
-
730343
Salmonella enterica subsp. enterica serovar Typhimurium
isocitrate
-
-
-
?
4.2.1.3
citrate
-
730343
Salmonella enterica subsp. enterica serovar Typhimurium
cis-aconitate
-
-
-
?
4.2.1.3
isocitrate
-
730343
Salmonella enterica subsp. enterica serovar Typhimurium
cis-aconitate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.3
monomer
gel filtration
Salmonella enterica subsp. enterica serovar Typhimurium
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.3
25
-
assay at
Salmonella enterica subsp. enterica serovar Typhimurium
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.3
1.1
-
isocitrate
pH 8, 25°C, Lineweaver-Burk method
Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.3
5.2
-
cis-aconitate
pH 8, 25°C, Lineweaver-Burk method
Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.3
5.3
-
citrate
pH 8, 25°C, Lineweaver-Burk method
Salmonella enterica subsp. enterica serovar Typhimurium
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.3
8
-
assay at
Salmonella enterica subsp. enterica serovar Typhimurium
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.3
expressed in Escherichia coli
Salmonella enterica subsp. enterica serovar Typhimurium
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.3
0.2
-
cis-aconitate
pH 8, 25°C, Lineweaver-Burk method
Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.3
0.9
-
isocitrate
pH 8, 25°C, Lineweaver-Burk method
Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.3
5.3
-
citrate
pH 8, 25°C, Lineweaver-Burk method
Salmonella enterica subsp. enterica serovar Typhimurium
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.3
using Ni-NTA chromatography
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
cis-aconitate
-
730343
Salmonella enterica subsp. enterica serovar Typhimurium
isocitrate
-
-
-
?
4.2.1.3
citrate
-
730343
Salmonella enterica subsp. enterica serovar Typhimurium
cis-aconitate
-
-
-
?
4.2.1.3
isocitrate
-
730343
Salmonella enterica subsp. enterica serovar Typhimurium
cis-aconitate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.3
monomer
gel filtration
Salmonella enterica subsp. enterica serovar Typhimurium
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.3
25
-
assay at
Salmonella enterica subsp. enterica serovar Typhimurium
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.3
1.1
-
isocitrate
pH 8, 25°C, Lineweaver-Burk method
Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.3
5.2
-
cis-aconitate
pH 8, 25°C, Lineweaver-Burk method
Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.3
5.3
-
citrate
pH 8, 25°C, Lineweaver-Burk method
Salmonella enterica subsp. enterica serovar Typhimurium
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.3
8
-
assay at
Salmonella enterica subsp. enterica serovar Typhimurium
Expression
EC Number
Organism
Commentary
Expression
4.2.1.3
Salmonella enterica subsp. enterica serovar Typhimurium
expression of acnA is repressed by the presence of acnB
down
4.2.1.3
Salmonella enterica subsp. enterica serovar Typhimurium
promoter fusion experiments show that acnA expression is regulated by the cyclic-AMP receptor protein, the fumarate nitrate reduction regulator, the ferric uptake regulator and the superoxide response protein
additional information
4.2.1.3
Salmonella enterica subsp. enterica serovar Typhimurium
acnA expression is enhanced in an acnB mutant
up
General Information
EC Number
General Information
Commentary
Organism
4.2.1.3
malfunction
acnA expression is enhanced in an acnB mutant
Salmonella enterica subsp. enterica serovar Typhimurium
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.2.1.3
malfunction
acnA expression is enhanced in an acnB mutant
Salmonella enterica subsp. enterica serovar Typhimurium
Expression (protein specific)
EC Number
Organism
Commentary
Expression
4.2.1.3
Salmonella enterica subsp. enterica serovar Typhimurium
expression of acnA is repressed by the presence of acnB
down
4.2.1.3
Salmonella enterica subsp. enterica serovar Typhimurium
promoter fusion experiments show that acnA expression is regulated by the cyclic-AMP receptor protein, the fumarate nitrate reduction regulator, the ferric uptake regulator and the superoxide response protein
additional information
4.2.1.3
Salmonella enterica subsp. enterica serovar Typhimurium
acnA expression is enhanced in an acnB mutant
up
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.2.1.3
1
-
citrate
pH 8, 25°C, Lineweaver-Burk method
Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.3
1.2
-
isocitrate
pH 8, 25°C, Lineweaver-Burk method
Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.3
26
-
cis-aconitate
pH 8, 25°C, Lineweaver-Burk method
Salmonella enterica subsp. enterica serovar Typhimurium
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.2.1.3
1
-
citrate
pH 8, 25°C, Lineweaver-Burk method
Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.3
1.2
-
isocitrate
pH 8, 25°C, Lineweaver-Burk method
Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.3
26
-
cis-aconitate
pH 8, 25°C, Lineweaver-Burk method
Salmonella enterica subsp. enterica serovar Typhimurium