BRENDA - Enzyme Database

Identification of binding sites of Lactobacillus plantarum enolase involved in the interaction with human plasminogen

Vastano, V.; Capri, U.; Candela, M.; Siciliano, R.A.; Russo, L.; Renda, M.; Sacco, M.; Microbiol. Res. 168, 65-72 (2013)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli BL21 cells
Lactobacillus plantarum
Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
K255A
while the binding activity of the mutated protein is drastically reduced, the residual enzymatic activity is more than 50% of the wild type enzyme
Lactobacillus plantarum
4.2.1.11
K259A K255A
while the binding activity of the mutated protein is drastically reduced, the residual enzymatic activity is more than 50% of the wild type enzyme
Lactobacillus plantarum
4.2.1.11
K259A/K422A K255A
while the binding activity of the mutated protein is drastically reduced, the residual enzymatic activity is more than 50% of the wild type enzyme
Lactobacillus plantarum
4.2.1.11
K422A K255A
while the binding activity of the mutated protein is drastically reduced, the residual enzymatic activity is more than 50% of the wild type enzyme
Lactobacillus plantarum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
48000
-
x * 48000, MALDI-TOF mass spectrometry
Lactobacillus plantarum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Lactobacillus plantarum
-
phosphoenolpyruvate + H2O
-
-
?
4.2.1.11
2-phospho-D-glycerate
Lactobacillus plantarum LM3
-
phosphoenolpyruvate + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Lactobacillus plantarum
-
-
-
4.2.1.11
Lactobacillus plantarum LM3
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
730339
Lactobacillus plantarum
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
-
730339
Lactobacillus plantarum LM3
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
additional information
the enzyme binds plasminogen
730339
Lactobacillus plantarum
?
-
-
-
-
4.2.1.11
additional information
the enzyme binds plasminogen
730339
Lactobacillus plantarum LM3
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 48000, MALDI-TOF mass spectrometry
Lactobacillus plantarum
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.2.1.11
Lactobacillus plantarum
isoelectric focusing
-
4.6
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli BL21 cells
Lactobacillus plantarum
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
K255A
while the binding activity of the mutated protein is drastically reduced, the residual enzymatic activity is more than 50% of the wild type enzyme
Lactobacillus plantarum
4.2.1.11
K259A K255A
while the binding activity of the mutated protein is drastically reduced, the residual enzymatic activity is more than 50% of the wild type enzyme
Lactobacillus plantarum
4.2.1.11
K259A/K422A K255A
while the binding activity of the mutated protein is drastically reduced, the residual enzymatic activity is more than 50% of the wild type enzyme
Lactobacillus plantarum
4.2.1.11
K422A K255A
while the binding activity of the mutated protein is drastically reduced, the residual enzymatic activity is more than 50% of the wild type enzyme
Lactobacillus plantarum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
48000
-
x * 48000, MALDI-TOF mass spectrometry
Lactobacillus plantarum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Lactobacillus plantarum
-
phosphoenolpyruvate + H2O
-
-
?
4.2.1.11
2-phospho-D-glycerate
Lactobacillus plantarum LM3
-
phosphoenolpyruvate + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
730339
Lactobacillus plantarum
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
-
730339
Lactobacillus plantarum LM3
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
additional information
the enzyme binds plasminogen
730339
Lactobacillus plantarum
?
-
-
-
-
4.2.1.11
additional information
the enzyme binds plasminogen
730339
Lactobacillus plantarum LM3
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 48000, MALDI-TOF mass spectrometry
Lactobacillus plantarum
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.2.1.11
Lactobacillus plantarum
isoelectric focusing
-
4.6