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Literature summary extracted from

  • Nygard, Y.; Maaheimo, H.; Mojzita, D.; Toivari, M.; Wiebe, M.; Resnekov, O.; Gustavo Pesce, C.; Ruohonen, L.; Penttilae, M.
    Single cell and in vivo analyses elucidate the effect of xylC lactonase during production of D-xylonate in Saccharomyces cerevisiae (2014), Metab. Eng., 25, 238-247.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.175 gene xylB, recombinnat expression in Saccharomyces cerevisiae strain VVT B-67002 Caulobacter vibrioides
3.1.1.68 gene xylC, functional expression in Saccharomyces cerevisiae, co-expression with D-xylose dehydrogenase, gene xylB, product determinations by NMR spectroscopy and pHluorin fluorescence measurement Caulobacter vibrioides

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.175 additional information both D-xylono-gamma-lactone and D-xylonate are produced when the Caulobacter crescentus gene xylB encoding D-xylose dehydrogenase is expressed in Saccharomyces cerevisiae, with or without co-expressionof xylC (D-xylonolactonase). XylC facilitates rapid opening of the lactone and more D-xylonate is initially produced than in its absence. In vivo [1H]NMR analysis of cell extracts, culture media and intact cells is used for analysis. The lactone and linear forms of D-xylonic acid are produced, accumulated intracellularly, and partially exported within 15–60 min after D-xylose provision. Co-expression of xylB and xylC leads to rapid loss of pHluorin fluorescence and loss of vitality during production of D-xylonate. Loss of vitality in the presence of D-xylose is correlated to the extracellular pH, but fluorescence is lost from xylB and xylC expressing cells regardless of the extracellular condition. Method optimization, overview Caulobacter vibrioides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.175 D-xylose + NAD+ Caulobacter vibrioides
-
 D-xylonolactone + NADH + H+
-
 ?
1.1.1.175 D-xylose + NAD+ Caulobacter vibrioides NA1000
-
 D-xylonolactone + NADH + H+
-
 ?
3.1.1.68 D-xylono-1,4-lactone + H2O Caulobacter vibrioides
-
 D-xylonate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.175 Caulobacter vibrioides B8H1Z0
-
-
1.1.1.175 Caulobacter vibrioides NA1000 B8H1Z0
-
-
3.1.1.68 Caulobacter vibrioides
-
 gene xylC
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.175 D-xylose + NAD+
-
 Caulobacter vibrioides D-xylonolactone + NADH + H+
-
 ?
1.1.1.175 D-xylose + NAD+
-
 Caulobacter vibrioides NA1000 D-xylonolactone + NADH + H+
-
 ?
3.1.1.68 D-xylono-1,4-lactone + H2O
-
 Caulobacter vibrioides D-xylonate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.175 D-xylose 1-dehydrogenase
-
 Caulobacter vibrioides
1.1.1.175 XylB
-
 Caulobacter vibrioides
3.1.1.68 D-xylonolactonase
-
 Caulobacter vibrioides
3.1.1.68 XylC
-
 Caulobacter vibrioides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.175 30
-
 in vivo assay, cell growth at 30°C Caulobacter vibrioides
3.1.1.68 22
-
 assay at Caulobacter vibrioides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.175 6.8
-
 assay at Caulobacter vibrioides
3.1.1.68 6.8
-
 assay at Caulobacter vibrioides

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.175 NAD+
-
 Caulobacter vibrioides