EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.2.1 | expression in Escherichia coli | Methanosarcina thermophila |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.2.1 | G239A | 14.5fold reduced specific activity (with ATP as substrate). The wild type enzyme shows broad NTP utilization, with greater than 50% activity with CTP, GTP, TTP, UTP, and ITP versus ATP. The mutant enzyme shows a shift in NTP utilization. It displays substantially higher activity with TTP than with ATP, and activity (as a percentage of that observed with ATP) increased greatly with CTP as well. A weak increase in activity is observed with UTP. Activity with the purines GTP and ITP versus ATP decreases | Methanosarcina thermophila |
2.7.2.1 | G239S | 192fold reduced specific activity (with ATP as substrate). The wild type enzyme shows broad NTP utilization, with greater than 50% activity with CTP, GTP, TTP, UTP, and ITP versus ATP. The mutant enzyme shows a shift in NTP utilization. It displays substantially higher activity with TTP than with ATP, and activity (as a percentage of that observed with ATP) increased greatly with CTP as well. A weak increase in activity is observed with UTP. Activity with the purines GTP and ITP versus ATP decreases | Methanosarcina thermophila |
2.7.2.1 | G331A | 15.2fold reduced specific activity (with ATP as substrate). The wild type enzyme shows broad NTP utilization, with greater than 50% activity with CTP, GTP, TTP, UTP, and ITP versus ATP. Activity of mutant enzyme with the pyrimidine nucleotides CTP, TTP, and UTP is significantly reduced, with each displaying less than 12% activity versus ATP. Activity with GTP and ITP is also reduced, but to a much lesser extent | Methanosarcina thermophila |
2.7.2.1 | G331Q | 118fold reduced specific activity. The wild type enzyme shows broad NTP utilization, with greater than 50% activity with CTP, GTP, TTP, UTP, and ITP versus ATP. Activity of mutant enzyme with the pyrimidine nucleotides CTP, TTP, and UTP is significantly reduced, with each displaying less than 12% activity versus ATP. Activity with GTP and ITP is also reduced, but to a much lesser extent | Methanosarcina thermophila |
2.7.2.1 | N211A | 6fold reduced specific activity (with ATP as substrate). The percentage activity observed with CTP and ITP versus ATP is similar to that observed with the wild type enzyme. Activity with GTP and UTP decreases somewhat, and activity with TTP shows an increase | Methanosarcina thermophila |
2.7.2.1 | N211S | 200fold reduced specific activity (with ATP as substrate). The percentage activity observed with CTP and ITP versus ATP is similar to that observed with the wild type enzyme. Activity with GTP and UTP decreases somewhat, and activity with TTP shows an increase | Methanosarcina thermophila |
2.7.2.1 | N211T | 44fold reduced specific activity (with ATP as substrate). Mutant enzyme shows little change in percentage activity observed with CTP and ITP. Activity with TTP is greatly enhanced and nearly equal to that observed with ATP, whereas the reduction in activity with UTP is stronger than that observed with the N211A | Methanosarcina thermophila |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.2.1 | 1.7 | - |
ATP | 37°C, pH 7.0-7.5, mutant enzyme G239A | Methanosarcina thermophila | |
2.7.2.1 | 1.8 | - |
ATP | 37°C, pH 7.0-7.5, mutant enzyme G239S | Methanosarcina thermophila | |
2.7.2.1 | 2.7 | - |
UTP | 37°C, pH 7.0-7.5, wild-type enzyme | Methanosarcina thermophila | |
2.7.2.1 | 2.7 | - |
TTP | 37°C, pH 7.0-7.5, wild-type enzyme | Methanosarcina thermophila | |
2.7.2.1 | 3.2 | - |
CTP | 37°C, pH 7.0-7.5, wild-type enzyme | Methanosarcina thermophila | |
2.7.2.1 | 4 | - |
ATP | 37°C, pH 7.0-7.5, wild-type enzyme | Methanosarcina thermophila | |
2.7.2.1 | 4.7 | - |
ITP | 37°C, pH 7.0-7.5, wild-type enzyme | Methanosarcina thermophila | |
2.7.2.1 | 5.3 | - |
GTP | 37°C, pH 7.0-7.5, mutant enzyme G331A | Methanosarcina thermophila | |
2.7.2.1 | 5.9 | - |
ATP | 37°C, pH 7.0-7.5, mutant enzyme N211A | Methanosarcina thermophila | |
2.7.2.1 | 6.8 | - |
ATP | 37°C, pH 7.0-7.5, mutant enzyme N211S | Methanosarcina thermophila | |
2.7.2.1 | 7.1 | - |
ATP | 37°C, pH 7.0-7.5, mutant enzyme N211T | Methanosarcina thermophila | |
2.7.2.1 | 7.2 | - |
GTP | 37°C, pH 7.0-7.5, wild-type enzyme | Methanosarcina thermophila | |
2.7.2.1 | 7.4 | - |
GTP | 37°C, pH 7.0-7.5, mutant enzyme G331Q | Methanosarcina thermophila | |
2.7.2.1 | 10.1 | - |
ITP | 37°C, pH 7.0-7.5, mutant enzyme G331Q | Methanosarcina thermophila | |
2.7.2.1 | 10.2 | - |
ATP | 37°C, pH 7.0-7.5, mutant enzyme G331A | Methanosarcina thermophila | |
2.7.2.1 | 10.7 | - |
ITP | 37°C, pH 7.0-7.5, mutant enzyme G331A | Methanosarcina thermophila | |
2.7.2.1 | 11.1 | - |
CTP | 37°C, pH 7.0-7.5, mutant enzyme G331Q | Methanosarcina thermophila | |
2.7.2.1 | 11.2 | - |
TTP | 37°C, pH 7.0-7.5, mutant enzyme G331A | Methanosarcina thermophila | |
2.7.2.1 | 12.1 | - |
TTP | 37°C, pH 7.0-7.5, mutant enzyme G331Q | Methanosarcina thermophila | |
2.7.2.1 | 14.2 | - |
UTP | 37°C, pH 7.0-7.5, mutant enzyme G331Q | Methanosarcina thermophila | |
2.7.2.1 | 14.3 | - |
acetate | 37°C, pH 7.0-7.5, wild-type enzyme | Methanosarcina thermophila | |
2.7.2.1 | 15.7 | - |
CTP | 37°C, pH 7.0-7.5, mutant enzyme G331A | Methanosarcina thermophila | |
2.7.2.1 | 18.4 | - |
ATP | 37°C, pH 7.0-7.5, mutant enzyme G331Q | Methanosarcina thermophila | |
2.7.2.1 | 94 | - |
acetate | 37°C, pH 7.0-7.5, mutant enzyme G239S | Methanosarcina thermophila | |
2.7.2.1 | 113 | - |
acetate | 37°C, pH 7.0-7.5, mutant enzyme G239A | Methanosarcina thermophila | |
2.7.2.1 | 140 | - |
acetate | 37°C, pH 7.0-7.5, mutant enzyme N211S | Methanosarcina thermophila | |
2.7.2.1 | 165 | - |
acetate | 37°C, pH 7.0-7.5, mutant enzyme N211A | Methanosarcina thermophila | |
2.7.2.1 | 470 | - |
acetate | 37°C, pH 7.0-7.5, mutant enzyme N211T | Methanosarcina thermophila | |
2.7.2.1 | 601 | - |
acetate | 37°C, pH 7.0-7.5, mutant enzyme G331A | Methanosarcina thermophila |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.2.1 | Methanosarcina thermophila | P38502 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.2.1 | - |
Methanosarcina thermophila |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.2.1 | ATP + acetate | the wild type enzyme shows broad NTP utilization, with greater than 50% activity with CTP, GTP, TTP, UTP, and ITP versus ATP | Methanosarcina thermophila | ADP + acetyl phosphate | - |
? | |
2.7.2.1 | CTP + acetate | 50% of the activity relative to that observed with ATP | Methanosarcina thermophila | CDP + acetyl phosphate | - |
? | |
2.7.2.1 | GTP + acetate | 85.2% of the activity relative to that observed with ATP | Methanosarcina thermophila | GDP + acetyl phosphate | - |
? | |
2.7.2.1 | ITP + acetate | 80% of the activity relative to that observed with ATP | Methanosarcina thermophila | IDP + acetyl phosphate | - |
? | |
2.7.2.1 | TTP + acetate | 64.5% of the activity relative to that observed with ATP | Methanosarcina thermophila | TDP + acetyl phosphate | - |
? | |
2.7.2.1 | UTP + acetate | 54.8% of the activity relative to that observed with ATP | Methanosarcina thermophila | UDP + acetyl phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.2.1 | ACK | - |
Methanosarcina thermophila |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.2.1 | 37 | - |
assay at | Methanosarcina thermophila |
2.7.2.1 | 65 | - |
- |
Methanosarcina thermophila |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.2.1 | 2.1 | - |
CTP | 37°C, pH 7.0-7.5, mutant enzyme G331Q | Methanosarcina thermophila | |
2.7.2.1 | 2.8 | - |
UTP | 37°C, pH 7.0-7.5, mutant enzyme G331Q | Methanosarcina thermophila | |
2.7.2.1 | 2.8 | - |
TTP | 37°C, pH 7.0-7.5, mutant enzyme G331Q | Methanosarcina thermophila | |
2.7.2.1 | 6.8 | - |
ATP | 37°C, pH 7.0-7.5, mutant enzyme G239S | Methanosarcina thermophila | |
2.7.2.1 | 8.6 | - |
GTP | 37°C, pH 7.0-7.5, mutant enzyme G331Q | Methanosarcina thermophila | |
2.7.2.1 | 11 | - |
ITP | 37°C, pH 7.0-7.5, mutant enzyme G331Q | Methanosarcina thermophila | |
2.7.2.1 | 13 | - |
ATP | 37°C, pH 7.0-7.5, mutant enzyme N211S | Methanosarcina thermophila | |
2.7.2.1 | 16 | - |
ATP | 37°C, pH 7.0-7.5, mutant enzyme G331Q | Methanosarcina thermophila | |
2.7.2.1 | 17 | - |
CTP | 37°C, pH 7.0-7.5, mutant enzyme G331A | Methanosarcina thermophila | |
2.7.2.1 | 20 | - |
TTP | 37°C, pH 7.0-7.5, mutant enzyme G331A | Methanosarcina thermophila | |
2.7.2.1 | 32 | - |
ATP | 37°C, pH 7.0-7.5, mutant enzyme N211T | Methanosarcina thermophila | |
2.7.2.1 | 43 | - |
UTP | 37°C, pH 7.0-7.5, mutant enzyme G331A | Methanosarcina thermophila | |
2.7.2.1 | 67 | - |
GTP | 37°C, pH 7.0-7.5, mutant enzyme G331A | Methanosarcina thermophila | |
2.7.2.1 | 77 | - |
ATP | 37°C, pH 7.0-7.5, mutant enzyme G239A | Methanosarcina thermophila | |
2.7.2.1 | 84 | - |
ITP | 37°C, pH 7.0-7.5, mutant enzyme G331A | Methanosarcina thermophila | |
2.7.2.1 | 88 | - |
ATP | 37°C, pH 7.0-7.5, mutant enzyme G331A | Methanosarcina thermophila | |
2.7.2.1 | 374 | - |
ATP | 37°C, pH 7.0-7.5, mutant enzyme N211A | Methanosarcina thermophila | |
2.7.2.1 | 415 | - |
UTP | 37°C, pH 7.0-7.5, wild-type enzyme | Methanosarcina thermophila | |
2.7.2.1 | 460 | - |
CTP | 37°C, pH 7.0-7.5, wild-type enzyme | Methanosarcina thermophila | |
2.7.2.1 | 540 | - |
TTP | 37°C, pH 7.0-7.5, wild-type enzyme | Methanosarcina thermophila | |
2.7.2.1 | 571 | - |
GTP | 37°C, pH 7.0-7.5, wild-type enzyme | Methanosarcina thermophila | |
2.7.2.1 | 715 | - |
ATP | 37°C, pH 7.0-7.5, wild-type enzyme | Methanosarcina thermophila | |
2.7.2.1 | 742 | - |
ITP | 37°C, pH 7.0-7.5, wild-type enzyme | Methanosarcina thermophila |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.2.1 | 0.2 | - |
CTP | 37°C, pH 7.0-7.5, mutant enzyme G331Q | Methanosarcina thermophila | |
2.7.2.1 | 0.2 | - |
UTP | 37°C, pH 7.0-7.5, mutant enzyme G331Q | Methanosarcina thermophila | |
2.7.2.1 | 0.2 | - |
TTP | 37°C, pH 7.0-7.5, mutant enzyme G331Q | Methanosarcina thermophila | |
2.7.2.1 | 0.9 | - |
ATP | 37°C, pH 7.0-7.5, mutant enzyme G331Q | Methanosarcina thermophila | |
2.7.2.1 | 1 | - |
ITP | 37°C, pH 7.0-7.5, mutant enzyme G331Q | Methanosarcina thermophila | |
2.7.2.1 | 1.1 | - |
CTP | 37°C, pH 7.0-7.5, mutant enzyme G331A | Methanosarcina thermophila | |
2.7.2.1 | 1.2 | - |
GTP | 37°C, pH 7.0-7.5, mutant enzyme G331Q | Methanosarcina thermophila | |
2.7.2.1 | 1.8 | - |
TTP | 37°C, pH 7.0-7.5, mutant enzyme G331A | Methanosarcina thermophila | |
2.7.2.1 | 1.9 | - |
ATP | 37°C, pH 7.0-7.5, mutant enzyme N211S | Methanosarcina thermophila | |
2.7.2.1 | 3.9 | - |
ATP | 37°C, pH 7.0-7.5, mutant enzyme G239S | Methanosarcina thermophila | |
2.7.2.1 | 4.5 | - |
ATP | 37°C, pH 7.0-7.5, mutant enzyme N211T | Methanosarcina thermophila | |
2.7.2.1 | 7.8 | - |
ITP | 37°C, pH 7.0-7.5, mutant enzyme G331A | Methanosarcina thermophila | |
2.7.2.1 | 8.9 | - |
ATP | 37°C, pH 7.0-7.5, mutant enzyme G331A | Methanosarcina thermophila | |
2.7.2.1 | 12.6 | - |
GTP | 37°C, pH 7.0-7.5, mutant enzyme G331A | Methanosarcina thermophila | |
2.7.2.1 | 45.7 | - |
ATP | 37°C, pH 7.0-7.5, mutant enzyme G239A | Methanosarcina thermophila | |
2.7.2.1 | 63 | - |
ATP | 37°C, pH 7.0-7.5, mutant enzyme N211A | Methanosarcina thermophila | |
2.7.2.1 | 80 | - |
GTP | 37°C, pH 7.0-7.5, wild-type enzyme | Methanosarcina thermophila | |
2.7.2.1 | 148 | - |
CTP | 37°C, pH 7.0-7.5, wild-type enzyme | Methanosarcina thermophila | |
2.7.2.1 | 152 | - |
UTP | 37°C, pH 7.0-7.5, wild-type enzyme | Methanosarcina thermophila | |
2.7.2.1 | 158 | - |
ITP | 37°C, pH 7.0-7.5, wild-type enzyme | Methanosarcina thermophila | |
2.7.2.1 | 180 | - |
ATP | 37°C, pH 7.0-7.5, wild-type enzyme | Methanosarcina thermophila | |
2.7.2.1 | 202 | - |
TTP | 37°C, pH 7.0-7.5, wild-type enzyme | Methanosarcina thermophila |