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Literature summary extracted from

  • Li, R.; Yu, Q.; Han, L.; Rong, L.; Yang, M.; An, M.
    Isolation and enzymatic characterization of the first reported hyaluronidase from Yak (Bos grunniens) testis (2014), Korean J. Chem. Engin., 31, 2027-2034.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.1.35 adding bovine serum albumin (1.0 mg/ml) to the enzyme sample prior to freezing results in complete retention of enzyme activity Bos grunniens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.35 Ca2+ 65% residual activity at 50 mM Bos grunniens
3.2.1.35 EDTA complete inhibition at 10 mM Bos grunniens
3.2.1.35 Fe2+ 77% residual activity at 50 mM Bos grunniens
3.2.1.35 Fe3+ 20% residual activity at 50 mM Bos grunniens
3.2.1.35 heparin 90% inhibition at 1.5 mg/ml Bos grunniens
3.2.1.35 Mg2+ 46% residual activity at 50 mM Bos grunniens
3.2.1.35 additional information not affected by ascorbic acid, phenylmethylsulfonyl fluoride, dithiothreitol, glutathione (reduced), or L-cysteine Bos grunniens
3.2.1.35 NaCl 0.5 M causes 92% inhibition Bos grunniens
3.2.1.35 Urea complete inhibition at 10 mM Bos grunniens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.35 additional information not affected by Cu2+, Zn2+, and Co2+ Bos grunniens
3.2.1.35 NaCl maximum activity in the presence of 0.15 M NaCl Bos grunniens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.35 55000
-
 gel filtration Bos grunniens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.35 Bos grunniens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.35 SP Sepharose column chromatography and Sephacryl S-100 gel filtration Bos grunniens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.35 testis
-
 Bos grunniens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.35 0.85
-
 crude enzyme, at pH 3.8 and 27°C Bos grunniens
3.2.1.35 20.4
-
 after 24fold purification, at pH 3.8 and 27°C Bos grunniens

Storage Stability

EC Number Storage Stability Organism
3.2.1.35 22°C, 100 mM sodium acetate buffer (pH 3.8) containing 150 mM NaCl, 4 weeks, 58% activity retained Bos grunniens
3.2.1.35 4°C, 100 mM sodium acetate buffer (pH 3.8) containing 150 mM NaCl, 4 weeks, 92% activity retained Bos grunniens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.35 chondroitin 4-sulfate + H2O 28% activity compared to sodium hyaluronate Bos grunniens ?
-
 ?
3.2.1.35 chondroitin 6-sulfate + H2O 19% activity compared to sodium hyaluronate Bos grunniens ?
-
 ?
3.2.1.35 dermatan + H2O 7% activity compared to sodium hyaluronate Bos grunniens ?
-
 ?
3.2.1.35 sodium hyaluronate + H2O 100% activity Bos grunniens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.35 monomer 1 * 55000, SDS-PAGE Bos grunniens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.35 Hyal
-
 Bos grunniens
3.2.1.35 hyaluronidase
-
 Bos grunniens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.35 37
-
-
 Bos grunniens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.35 20 55 more than 50% activity between 20 and 55°C. Activity is abolished at 60°C Bos grunniens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.35 3.8
-
-
 Bos grunniens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.35 2.5 9 the optimum activity at pH 3.8 decreases by half at pH 2.5 and 6.0 and is virtually abolished at pH 9.0 Bos grunniens