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Literature summary extracted from

  • Sandeep, B.; Kishor Mohan, T.; Murugan, K.
    Purification and kinetic characterization of polygalacturonase-3 from palmyrah palm (Borassus flabellifer L) (2014), J. Plant Biochem. Biotechnol., 23, 221-224.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.15 2-mercaptoethanol slight activation at 0.1-0.5 mM Borassus flabellifer
3.2.1.15 PEG slight activation at 0.1 mM, no more at 0.5 mM Borassus flabellifer

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.15 Ba2+ 12% inhibition at 0.5 mM Borassus flabellifer
3.2.1.15 Cd2+ 98% inhibition at 0.5 mM Borassus flabellifer
3.2.1.15 cetyltrimethylammonium bromide 67% inhibition at 0.5% Borassus flabellifer
3.2.1.15 Cu2+ 23% inhibition at 0.5 mM Borassus flabellifer
3.2.1.15 Fe2+ 18% inhibition at 0.5 mM Borassus flabellifer
3.2.1.15 Hg2+ 96% inhibition at 0.5 mM Borassus flabellifer
3.2.1.15 p-chloromercuribenzoate 80% inhibition at 0.1 mM, 94% at 0.5 mM Borassus flabellifer
3.2.1.15 Pb2+ 97% inhibition at 0.5 mM Borassus flabellifer
3.2.1.15 Teepol 43% inhibition at 0.5% Borassus flabellifer
3.2.1.15 Triton X-100 10% inhibition at 0.5% Borassus flabellifer

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.15 0.33
-
 poly-D-galacturonic acid pH 5.0, 40°C Borassus flabellifer

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.15 Ca2+ activates 46% at 0.5 mM Borassus flabellifer
3.2.1.15 Mg2+ activates 10% at 0.5 mM Borassus flabellifer
3.2.1.15 additional information no effects by 0.5 mM of Mn2+, Na+, and K+ Borassus flabellifer

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.15 66000
-
 gel filtration Borassus flabellifer

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.15 Borassus flabellifer
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.15 native enzyme 100.9fold from palm fruits to homogeneity by concanavalin A affinity chromatography Borassus flabellifer

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.15 fruit
-
 Borassus flabellifer
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.15 322.9
-
 purified enzyme, pH 5.0, 40°C Borassus flabellifer

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.15 poly-D-galacturonic acid + H2O
-
 Borassus flabellifer D-galacturonic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.15 monomer 1 * 66000, about, SDS-PAGE Borassus flabellifer

Synonyms

EC Number Synonyms Comment Organism
3.2.1.15 PG-3
-
 Borassus flabellifer
3.2.1.15 polygalacturonase-3
-
 Borassus flabellifer

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.15 30 40
-
 Borassus flabellifer

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.15 60
-
 purified enzyme, pH 5.0, stable up to Borassus flabellifer
3.2.1.15 70
-
 purified enzyme, pH 5.0, loss of 52% activity Borassus flabellifer
3.2.1.15 80
-
 purified enzyme, pH 5.0, loss of 90% activity Borassus flabellifer

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.15 5
-
-
 Borassus flabellifer

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.15 4 9 activity range, 19% of maximal activity at pH 9.0 Borassus flabellifer