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Literature summary extracted from

  • Singh, A.; Chand, D.; Pattanayak, D.
    Purification and characterization of potato ribonuclease P (2013), J. Plant Biochem. Biotechnol., 22, 425-433.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.26.5 DNA and amino acid sequence determination and analysis of two cDNAs encoding two putative protein subunits of potato ribonuclease P, StPop5 and StRpp25, subcloning in Escherichia coli strain M15, recombinant overexpression as His6-tagged proteins in Escherichia coli strain BL21(DE3) Solanum tuberosum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.26.5 nucleus
-
Solanum tuberosum 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.5 Mg2+ required Solanum tuberosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.26.5 18000
-
x * 18000, recombinant His6-tagged protein StPop5, SDS-PAGE, x * 33000, recombinant His6-tagged protein StRpp25, SDS-PAGE Solanum tuberosum
3.1.26.5 33000
-
x * 18000, recombinant His6-tagged protein StPop5, SDS-PAGE, x * 33000, recombinant His6-tagged protein StRpp25, SDS-PAGE Solanum tuberosum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.26.5 additional information Solanum tuberosum the natural substrate is precursor tRNA ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.5 Solanum tuberosum
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.26.5 ribonucleoprotein
-
Solanum tuberosum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.5 native nuclear enzyme partially purified from leaves employing anion exchange chromatography, and from floral buds employing two different steps of anion exchange chromatography, ammonium sulfate fractionation, and gel filtration, purification of recombinant His6-tagged proteins StPop5 and StRpp25 from Escherichia coli strain BL21(DE3) Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.26.5 flower bud
-
Solanum tuberosum
-
3.1.26.5 leaf
-
Solanum tuberosum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.5 additional information the natural substrate is precursor tRNA Solanum tuberosum ?
-
?
3.1.26.5 additional information assay substrate is tobacco chloroplast precursor tRNAGly Solanum tuberosum ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.26.5 ? x * 18000, recombinant His6-tagged protein StPop5, SDS-PAGE, x * 33000, recombinant His6-tagged protein StRpp25, SDS-PAGE Solanum tuberosum

Synonyms

EC Number Synonyms Comment Organism
3.1.26.5 RNase P
-
Solanum tuberosum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.26.5 37
-
assay at Solanum tuberosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.26.5 8
-
assay at Solanum tuberosum

General Information

EC Number General Information Comment Organism
3.1.26.5 additional information the two protein subunits StPop5 and StRpp25 are associated with with floral bud enzyme activity but not with leaf enzyme activity Solanum tuberosum
3.1.26.5 physiological function the ribonucleoprotein endoribonuclease is responsible for 5' maturation of precursor tRNA Solanum tuberosum