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Literature summary extracted from

  • Tian, F.; Khodadadi, M.; Karboune, S.
    Optimization of levansucrase/endo-inulinase bi-enzymatic system for the production of fructooligosaccharides and oligolevans from sucrose (2014), J. Mol. Catal. B, 109, 85-93.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.7 synthesis application of a bi-enzymatic system based on the combined use of levansucrase from Bacillus amyloliquefaciens and endo-inulinase from Aspergillus niger in a one-step reaction for the synthesis of fructooligosaccharides and oligolevans using sucrose as the sole substrate. The optimal conditions leading to a high yield of short chain fructooligosaccharides, i.e.1:1 ratio, 0.5 h, 0.6 M, are different from those resulting in a high yield of medium chain fructooligosaccharides and oligolevans, i.e. 1.85:1 ratio, 1.77 h, 0.6 M. The production of fructooligosaccharides and oligolevans at a large scale gives a yield of 57-65%, w/w and produces 65.8-266.8 g/l and h, and uses of low temperature of 35°C and low concentrations of sucrose Aspergillus niger

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.10 additional information design and evaluation of a bi-enzymatic system: combined use of levansucrase from Bacillus amyloliquefaciens and endoinulinase from Aspergillus niger in a one-step reaction for the synthesis of fructooligosaccharides (FOSs) and oligolevans using sucrose as the sole substrate, overview Bacillus amyloliquefaciens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.10 extracellular
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 Bacillus amyloliquefaciens
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Organism

EC Number Organism UniProt Comment Textmining
2.4.1.10 Bacillus amyloliquefaciens P21130
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2.4.1.10 Bacillus amyloliquefaciens ATCC 23350 P21130
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3.2.1.7 Aspergillus niger
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.10 additional information design and evaluation of a bi-enzymatic system: combined use of levansucrase from Bacillus amyloliquefaciens and endoinulinase from Aspergillus niger in a one-step reaction for the synthesis of fructooligosaccharides (FOS) and oligolevans using sucrose as the sole substrate, overview. At the initial stage of time course, short chain fructooligosaccharides, 1-kestose, nystose, and 1F-fructosylnystose are the major products, whilst 6-kestose, medium chain fructooligosaccharides (mcFOSs, levanohexaose, levanopentaose) and oligolevans become the dominant ones at the late stage. NMR analysis of levan, levanopentaose and levanohexaose, overview Bacillus amyloliquefaciens ?
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 ?
2.4.1.10 additional information design and evaluation of a bi-enzymatic system: combined use of levansucrase from Bacillus amyloliquefaciens and endoinulinase from Aspergillus niger in a one-step reaction for the synthesis of fructooligosaccharides (FOS) and oligolevans using sucrose as the sole substrate, overview. At the initial stage of time course, short chain fructooligosaccharides, 1-kestose, nystose, and 1F-fructosylnystose are the major products, whilst 6-kestose, medium chain fructooligosaccharides (mcFOSs, levanohexaose, levanopentaose) and oligolevans become the dominant ones at the late stage. NMR analysis of levan, levanopentaose and levanohexaose, overview Bacillus amyloliquefaciens ATCC 23350 ?
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 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.10 SacB
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 Bacillus amyloliquefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.10 30
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 assay at Bacillus amyloliquefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.10 6
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 assay at Bacillus amyloliquefaciens