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Literature summary extracted from

  • Topal, H.; Fulcher, N.B.; Bitterman, J.; Salazar, E.; Buck, J.; Levin, L.R.; Cann, M.J.; Wolfgang, M.C.; Steegborn, C.
    Crystal structure and regulation mechanisms of the CyaB adenylyl cyclase from the human pathogen Pseudomonas aeruginosa (2012), J. Mol. Biol., 416, 271-286.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.1 expressed in Escherichia coli SG13009[pREP4] andM15[pREP4] cells Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.6.1.1 using 0.1 M 4-morpholineethanesulfonic acid (pH 6.5) and 12% (w/v) polyethylene glycol 20000 Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.1 A197T the mutant shows increased activity compared to the wild type enzyme Pseudomonas aeruginosa
4.6.1.1 E189R the mutant shows increased activity compared to the wild type enzyme Pseudomonas aeruginosa
4.6.1.1 E377G the mutant shows increased activity compared to the wild type enzyme Pseudomonas aeruginosa
4.6.1.1 F399H the mutant shows increased activity compared to the wild type enzyme Pseudomonas aeruginosa
4.6.1.1 F399I the mutant shows increased activity compared to the wild type enzyme Pseudomonas aeruginosa
4.6.1.1 I352T the mutant shows increased activity compared to the wild type enzyme Pseudomonas aeruginosa
4.6.1.1 L326P the mutant shows increased activity compared to the wild type enzyme Pseudomonas aeruginosa
4.6.1.1 R318W the mutant shows increased activity compared to the wild type enzyme Pseudomonas aeruginosa
4.6.1.1 R412H the mutant shows slightly increased activity compared to the wild type enzyme Pseudomonas aeruginosa
4.6.1.1 R456L the mutant shows strongly increased activity compared to the wild type enzyme Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.1 2-hydroxyestradiol significant inhibition at 0.1 mM Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.6.1.1 inner membrane
-
 Pseudomonas aeruginosa
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.6.1.1 40000
-
 2 * 40000, SDS-PAGE Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.1 ATP Pseudomonas aeruginosa
-
 3',5'-cyclic AMP + diphosphate
-
 ?
4.6.1.1 ATP Pseudomonas aeruginosa ATCC 15692
-
 3',5'-cyclic AMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.1 Pseudomonas aeruginosa Q9HZ23
-
-
4.6.1.1 Pseudomonas aeruginosa ATCC 15692 Q9HZ23
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.1 Ni-NTA agarose column chromatography and Superose 12 gel filtration Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.1 ATP
-
 Pseudomonas aeruginosa 3',5'-cyclic AMP + diphosphate
-
 ?
4.6.1.1 ATP
-
 Pseudomonas aeruginosa ATCC 15692 3',5'-cyclic AMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.6.1.1 homodimer 2 * 40000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
4.6.1.1 adenylyl cyclase
-
 Pseudomonas aeruginosa
4.6.1.1 CyaB
-
 Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
4.6.1.1 physiological function the enzyme is a key regulator of Pseudomonas aeruginosa virulence Pseudomonas aeruginosa