Literature summary extracted from
Morales-Alvarez, E.D.; Rivera-Hoyos, C.M.; Baena-Moncada, A.M.; Landazuri, P.; Poutou-Pinales, R.A.; Saenz-Suarez, H.; Barrera, L.A.; Echeverri-Pena, O.Y.
Low-scale expression and purification of an active putative iduronate 2-sulfate sulfatase-Like enzyme from Escherichia coli K12 (2013), J. Microbiol., 51, 213-221.
Application
EC Number |
Application |
Comment |
Organism |
---|
3.1.6.13 |
synthesis |
expression and purification of enzyme from Escherichia coli, strategy for improving protein expression and purification |
Homo sapiens |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.6.13 |
expression in Escherichia coli |
Homo sapiens |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.1.6.13 |
40000 |
- |
x * 40000, SDS-PAGE |
Homo sapiens |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.6.13 |
Homo sapiens |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.1.6.13 |
strategy for improving protein expression and purification of recombinant protein |
Homo sapiens |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
3.1.6.13 |
78 |
94 |
pH 5.0, 37°C |
Homo sapiens |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.6.13 |
4-methylumbelliferyl-alpha-iduronate 2-sulfate + H2O |
- |
Homo sapiens |
4-methylumbelliferyl-alpha-iduronate + sulfate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.1.6.13 |
? |
x * 40000, SDS-PAGE |
Homo sapiens |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.6.13 |
IDS-like enzyme |
- |
Homo sapiens |
3.1.6.13 |
iduronate 2-sulfate-like enzyme |
- |
Homo sapiens |